Sequences from confirmation amplicon


Confirmation sequences for line 851H06 and hit At5g15630 are shown.
Line ID 851H06
Locus At5g15630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5085761
Primers used for PCR AC74 - 8474, show primer details
   
Sequence Name 52-K104214-0022-851-H06C-AC74
GenBank Accession LN507805 [GenBank]
Sequencing primer gene specific primer, AC74: 5'-GCTGTAAGAAAACCCCTACAGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K104214-0022-851-H06C-AC74
TTATATCAACAGTTCTCAAACTGTTGCAAGGAGGTGTAATTGGAGCTTGGGGTCAAGATC
CATCAGCCGCTGTATCCCAGTTTCAGGTTAGTGCTGGTTTAGCTGGAACTACAAACAAGA
CTGTCAAGCTTCCTAAGAACTTCACTTTGCTTGGTCCCGGCCCTGGTTACACTTGCGGTC
CTGCCAAAATCGTGCCCTCTACCGTTTTTCTCACAACTGACAAACGGCGAAAAACACAAG
CTTTGAGTAAGCAATCAATCAAAAACCTTAACTTGTTCCATCATCGAAATCTAAACATCA
ATTTCAAACTTATGTTTCTGCAGTGACATGGAATGTTACCTGCACATACTCACAGTTTTT
AGCAAGAAAGCATCCAAGCTGTTGTGTCTCCTTCTCTTCTTTCTACAACGGCTTCAATTG
TAAATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCACGATTATTA
   
Sequence Name 12-K104215-0022-851-H06C-8409
GenBank Accession LN491555 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104215-0022-851-H06C-8409
GCCTTTACAATTGAAGCCTTTACAATTGAAGCCGTTGTAGAAAGAAGAGAAGGAGACACA
ACAGCTTGGATGCTTTCTTGCTAAAAACTGTGAGTATGTGCAGGTAACATTCCATGTCAC
TGCAGAAACATAAGTTTGAAATTGATGTTTAGATTTCGATGATGGAACAAGTTAAGGTTT
TTGATTGATTGCTTACTCAAAGCTTGTGTTTTTCGCCGTTTGTCAGTTGTGAGAAAAACG
GTAGAGGGCACGATTTTGGCAGGACCGCAAGTGTAACCAGGGCCGGGACCAAGCAAAGTG
AAGTTCTTAGGAAGCTTGACAGTCTTGTTTGTAGTTCCAGCTAAACCAGCACTAACCTGA
AACTGGGATACAGCGGCTGATGGATCTTGACCCCAAGCTCCAATTACACCTCCTTTGCAA
CAGTTTGAGAACTGTTGATTATAAGGCACACCTGGCAAGAGATCAACAACTGTAGGGGTT
TTCTTACAG

Last Updated on 06.12.2018 16:11