Sequences from confirmation amplicon


Confirmation sequences for line 852B07 and hit At1g17070 are shown.
Line ID 852B07
Locus At1g17070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5839322
Primers used for PCR 48bk - 8474, show primer details
   
Sequence Name 43-K107550-0022-852-B07C-48bk
GenBank Accession LN504857 [GenBank]
Sequencing primer gene specific primer, 48bk: 5'-GAAGAACCTCTCCATCATATCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K107550-0022-852-B07C-48bk
TTCTAACCCAATCAAACCGTTCTAATTCTGGTTTGCCGGGTTTACCTGAAACTCTTGCAA
AGCCAGCTGAAGTTTCGGAACTATGTAACGTCGCATAAGCTGTTCCCAGCTTGTAGTATC
AAAAACGGTCTTCCATGGTGAAAGAATAGTGTAGGCAGATGAATCACTGGGATGCCAAGC
ATCAAGAACATTACTCAATTTCATCTGAATGATCTGATAAAGAAACTCCAACTTCTGACC
AAGTATAGGCAACCAAGGATGCACCCAAACATGGATGGCAACAAGTTCCCTTCGTGGATC
CCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCC
   
Sequence Name 35-K107549-0022-852-B07C-8409
GenBank Accession LN501858 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K107549-0022-852-B07C-8409
ATTTACATTGAATATTGGGATCCCGAAGGGAACTTGTTGCCATCCATGTTTGGGTGCATC
CTTGGTTGCCTATACTTGGTCAGAAGTTGGAGTTTCTTTATCAGATCATTCAGATGAAAT
TGAGTAATGTTCTTGATGCTTGGCATCCCAGTGATTCATCTGCCTACACTATTCTTTCAC
CATGGAAGACCGTTTTTGATACTACAAGCTGGGAACAGCTTATGCGACGTTACATAGTTC
CGAAACTTCAGCTGGCTTTGCAAGAGTTTCAGGTAAACCCGGCAAACCAGAATTTAGAAC
GGTTTGATTGGGTTATGAAGTGGGCATCTGCAGTACCCATACATCTAATGGCTGATATGA
TGGAAGGGTTCTT

Last Updated on 06.12.2018 16:11