Sequences from confirmation amplicon


Confirmation sequences for line 852C11 and hit At1g07400 are shown.
Line ID 852C11
Locus At1g07400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2275521
Primers used for PCR BF45 - 8474, show primer details
   
Sequence Name 09-K029007-022-852-C11C-8409
GenBank Accession LN490044 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K029007-022-852-C11C-8409
TAGATTTCATGTAGATTATTTGATCCATCATTACTCTTTCTCCTTCTTCGGTTTCAAGGC
TTCCATGGAGAACGGAGTTCTGACTGTGACGGTGCCTAAAGTGGAAGAGGCTAAGAAGAA
GGCTCAAGTTAAGTCTATTGATATCTCTGGCTAAAGCCAATTAGCTTATGGGTTTCATGT
TTATGCGTAAAGACTCTTCTGATTATGGCTCTTGATTTGAATATGGCTCTGTGTTTTGGT
AATGATGTATTATAACTGTTAATCCTAATAATTAAGCAAGGCCTTTATATTCTCGTTTCA
ATTGCTTTTTCTGAAGATTTTAGCCGTAAATTCGATTTTAACTCGGATGGGACCAACTAA
ACTTAAGTTTAAAGGTTGAATTTTTAATTTAGATATGCAATTTGCATAATCTGATCTTAG
TTTTGGTATGACTGATTTGTAACAAAGTCTTATAACAAAAAGTTGAAATTTAATGAAATT
ATTTCTTCCGTGGTAAACAAAAAAAAAAGAATCTCAAAATTCGAAACTAAATAAAAGGGA
GTTTCCGTTTTCACCTCTTGGGCACAAAACCCGCAACGTATAAGGAAAGGAAACAGAGAA
ATACAGAAACAAAATATATAAAAAAAACATTTGTTCCATTCTCGATCCTTCCGGCTTGCT
CAAAAGGTAAAATTCGATTTTCTAGGGTTTCGATTTGCTGCTTTTTGATCTCATTGACGA
TGATTAGTTGTAGTTTCT
   
Sequence Name 10-K029007-022-852-C11C-BF45
GenBank Accession LN490518 [GenBank]
Sequencing primer gene specific primer, BF45: 5'-CAACATCGATCGAAGAAACTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K029007-022-852-C11C-BF45
AAGCAGCAAATCGAAACCCTAGAAAATCGAATTTTACCTTTTGAGCAAGCCGCAAGGATC
GAGAATGGAACAAATGTTTTTTTTATATATTTTGTTTCTGTATTTCTCTGTTTCCTTTCC
TTATACGTTGCGGGTTTTGTGCCCAAGAGGTGAAAACGGAAACTCCCTTTTATTTAGTTT
CGAATTTTGAGATTCTTTTTTTTTTGTTTACCACGGAAGAAATAATTTCATTAAATTTCA
ACTTTTTGTTATAAGACTTTGTTACAAATCAGTCATACCAAAACTAAGATCAGATTATGC
AAATTGCATATCTAAATTAAAAATTCAACCTTTAAACTTAAGTTTAGTTGGTCCCATCCG
AGTTAAAATCGAATTTACGGCTAAAATCTTCAGAAAAAGCAATTGAAACGAGAATATAAA
GGCCTTGCTTAATTATTAGGATTAACAGTTATAATACATCATTACCAAAACACAGAGCCA
TATTCAAATCAAGAGCCATAATCAGAAGAGTCTTTACGCATAAACATGAAACCCATAAGC
TAATTGGCTTTAGCCAGAGATATCAATAGACTTAACTTGAGCCTTCTTCTTAGCCTCTTC
CACTTTAGGCACCGTCACAGTCAGAACTCCGTTCTCCATGGAAGCCTTGAAACCGAAGAA
AAAGAAAGAGTAATGATGGATCAAATAATCTACATGAAATCTACATGGATCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAA

Last Updated on 06.12.2018 16:11