Sequences from confirmation amplicon


Confirmation sequences for line 853B10 and hit At3g05700 are shown.
Line ID 853B10
Locus At3g05700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1683714
Primers used for PCR KD46 - 8474, show primer details
   
Sequence Name 94-K034071-022-853-B10C-KD46
GenBank Accession LN515278 [GenBank]
Sequencing primer gene specific primer, KD46: 5'-GGCGAAGGTAGTTTAGGTGATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K034071-022-853-B10C-KD46
TTCTTTCGATTTAGATCTCTGTTTTGAAGTTAAGAAATATATGAGAAATCTTCGATCTTG
TTTTGAAAGATTTCGTATTTTGTCATTTTTTCATCGCCTTAGGCTTTGCTTGATACTAGC
AAAAATGAAGGAAAGGGGGCTTTTGTAATTGATTTTTATTGACTGATGAAATATGGTTTG
ATTCTGGTTTTGCTCGATTCCTGAGAAAATGGATGAAAATTATCCAAAGTTGCAGTCTTT
GTTAGCATTTTAATTACTCCTGTGATGCTACTATGCTCAGTGTTTATGAACATGAGAAAG
TGAAGATATTGTTCTCCTCAGAAGAGGAGAATATGTTTCATATACTGTAACCTCAGAGGT
AGAGTAGGGGTTTAGTATATCTGATTACTCTCTTTGTTTTCTTATAGCTTTGCTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACT
CCCCAAATGG
   
Sequence Name 67-K034071-022-853-B10C-8409
GenBank Accession LN511750 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K034071-022-853-B10C-8409
AAGCCATTTACAGCAAAGCTATAAGAAAACAAAGAGAGTAATCAGATATACTAAACCCCT
ACTCTACCTCTGAGGTTACAGTATATGAAACATATTCTCCTCTTCTGAGGAGAACAATAT
CTTCACTTTCTCATGTTCATAAACACTGAGCATAGTAGCATCACAGGAGTAATTAAAATG
CTAACAAAGACTGCAACTTTGGATAATTTTCATCCATTTTCTCAGGAATCGAGCAAAACC
AGAATCAAACCATATTTCATCAGTCAATAAAAATCAATTACAAAAGCCCCCTTTCCTTCA
TTTTTGCTAGTATCAAGCAAAGCCTAAGGCGATGAAAAAATGACAAAATACGAAATCTTT
CAAAACAAGATCGAAGATTTCTCATATATTTCTTAATCTTCAAAACAGAGATTCTAAATC
GGAAAGAAAACACAAGTCTATCACCTAACTACCCTTCG

Last Updated on 06.12.2018 16:11