Sequences from confirmation amplicon


Confirmation sequences for line 853H01 and hit At4g22100 are shown.
Line ID 853H01
Locus At4g22100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11709058
Primers used for PCR 23wb - 8474, show primer details
   
Sequence Name 69-K118393-0022-853-H01C-23wb
GenBank Accession LN512282 [GenBank]
Sequencing primer gene specific primer, 23wb: 5'-TTATTCACCAAGCAAACTCTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118393-0022-853-H01C-23wb
TGCACATCAGAGATTCTGCTTTTGCATGTGCAGGAGGATGTGAAGCTCATGGTGGAAACT
GGCTTAGATGCCTTCAGATTCTCCATTTCTTGGTCTAGGCTTATACCGAGTAAGAAGTCT
TCTTAAACACGCTTAATGTTTTCACGCCGGAACTTTACTTGATGAGCACTTATCTCATTT
AGATGGAAGAGGTCCTGTTAATCCCAAGGGTCTACAGTTCTATAAGAACTTCATTCAAGA
ACTTGTAAGCCATGGTGAGACTACTCATGGATACCAAAAACTTCTGTTGTGTTGAGTTTG
GACTTAAAGTAAACTTTTACAGGAATCGAACCACATGTGACACTGTTCCACTACGATCAT
CCTCAGTATCTTGAAGATGAATATGGAGGATAAATCATCTATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 21-K118392-0022-853-H01C-8409
GenBank Accession LN495863 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118392-0022-853-H01C-8409
GATGATTTATCCTCCATATTCATCTTCAAGATACTGAGGATGATCGTAGTGGAACAGTGT
CACATGTGGTTCGATTCCTGTAAAAGTTTACTTTAAGTCCAAACTCAACACAACAGAAGT
TTTTGGTATCCATGAGTAGTCTCACCATGGCTTACAAGTTCTTGAATGAAGTTCTTATAG
AACTGTAGACCCTTGGGATTAACAGGACCTCTTCCATCTAAATGAGATAAGTGCTCATCA
AGTAAAGTTCCGGCGTGAAAACATTAAGCGTGTTTAAGAAGACTTCTTACTCGGTATAAG
CCTAGACCAAGAAATGGAGAATCTGAAGGCATCTAAGCCAGTTTCCACCATGAGCTTCAC
ATCCTCCTGCACATGCAAAAGCAGAATCTCTGATGTGCAATAACAAAGCCAATGTTTCTG
TAATTAACTATTCAAGAGTTTGCTGGGNTGAATA

Last Updated on 06.12.2018 16:11