Sequences from confirmation amplicon


Confirmation sequences for line 854B10 and hit At5g50330 are shown.
Line ID 854B10
Locus At5g50330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20488365
Primers used for PCR 40uj - 8474, show primer details
   
Sequence Name 32-K117387-0022-854-B10C-40uj
GenBank Accession LN500741 [GenBank]
Sequencing primer gene specific primer, 40uj: 5'-AATGTTGCTACAGTTCCATGTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K117387-0022-854-B10C-40uj
TTCTAGCTATTCAGAAACCATCATAACCAGATTATGTTCCTCTTTTCCTAATGTTCCATA
ATACTCAAACTCAATCATGCTTGCTTAATTGTAAGGTTTCAACCAAAATCTGTAATACCT
GAGCAATAGAAGCAGAACCAAGAGGCTTTTCATCAAACGTTTCAAAGATTTCACCAATGC
TTTTACCTAACTCCTTCTCTAGAACAAGTTGAATCGCATCAAACGGCGTCGCAGGAGCTT
GATCACACAAAGTGACAAGCTTTTTCACCCACGCAGCTGGTGCCATGTCAGGTTTTGCTA
AAAGTTGAGCAATCTATTTAACCATTCAAACAAAAAAAGTCAAACATCAACAGAACACAA
CAACAACCATTAATGCAATAAAGCCAAACACTTTAAGTACCTTAAGGAAGAAACCACCAA
GATCAGAGCACATGAAGTAAATCTTATCAGCAGCTTGTTCATGTTGTCTTTCCCACATTT
CCTCTTGCTTCTTTGCATCTTTTACTAAACTTACTCGAAGTTGAAACACCTGAATCCAAA
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTA
   
Sequence Name 05-K117386-0022-854-B10C-8409
GenBank Accession LN488528 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117386-0022-854-B10C-8409
ATTTCATTGAATATATCCTGTTTGGATTCAGGTGTTTCAACTTCGAGTAAGTTTAGTAAA
AGATGCAAAGAAGCAAGAGGAAATGTGGGAAAGACAACATGAACAAGCTGCTGATAAGAT
TTACTTCATGTGCTCTGATCTTGGTGGTTTCTTCCTTAAGGTACTTAAAGTGTTTGGCTT
TATTGCATTAATGGTTGTTGTTGTGTTCTGTTGATGTTTGACTTTTTTTGTTTGAATGGT
TAAATAGATTGCTCAACTTTTAGCAAAACCTGACATGGCACCAGCTGCGTGGGTGAAAAA
GCTTGTCACTTTGTGTGATCAAGCTCCTGCGACGCCGTTTGATGCGATTCAACTTGTTCT
AGAGAAGGAGTTAGGTAAAAGCATTGGTGAAATCTTTGAAACGTTTGATGAAAAGCCTCT
TGGTTCTGCTTCTATTGCTCAGGTATTACAGATTTTGGTTGAAACCTTACAATTAAGCAA
GCATGATTGAGTTTGAGTATTATGGAACATTAGGAAAAGAGGAACATAATCTGGTTATGA
TGGTTTCTTGAATTAGCTAGAACCTTGTCCTCTCTTCCCATAGAATTATTATCTCCTGAC
ATGGAACTTAACCAACA

Last Updated on 06.12.2018 16:11