Sequences from confirmation amplicon


Confirmation sequences for line 855B01 and hit At1g10490 are shown.
Line ID 855B01
Locus At1g10490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3459722
Primers used for PCR 66gp - 8474, show primer details
   
Sequence Name 56-K112131-0022-855-B01C-66gp
GenBank Accession LN509145 [GenBank]
Sequencing primer gene specific primer, 66gp: 5'-AACCAGTTTCACACATTGCAGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K112131-0022-855-B01C-66gp
GAACTTTGTATATGGTTGTAAATGCTCAAACAGAATCTGTAAGTTTTTTTAAAAAAATTG
TATGATCAAACCATAATTTTAATAAATTATTCAACCTGGTTACAAAAATTCGTAAAAAGA
CCAAAGATCAAAATTTTCTGGTCACTGTTACAAAAAAACAATTGTTGACATCAACCTCCA
TCTCAGGCTCTTCTCTTTTTGTTGGATGTGGAGGAATGCTTATCATTGCCTCTCTTTTTG
GCTGATTTATCAAAACCACTTGGCTTCTCTTTCTTGTTATCAGTTTTGGTGGACTCTATA
CTGATAAGACCACTTGAAGAGATCTTCGACTTTTGCAAAGCCTCAGCTTCTTTGTCCCCA
ATAGCAAATTGGTCAAGTAGCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 40-K112130-0022-855-B01C-8409
GenBank Accession LN503750 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K112130-0022-855-B01C-8409
GAGCATTTCATTGAATATATCCTGAGCTACTTGACCAATTTGCTATTGGGGACAAAGAAG
CTGAGGCTTTGCAAAAGTCGAAGATCTCTTCAAGTGGTCTTATCAGTATAGAGTCCACCA
AAACTGATAACAAGAAAGAGAAGCCAAGTGGTTTTGATAAATCAGCCAAAAAGAGAGGCA
ATGATAAGCATTCCTCCACATCCAACAAAAAGAGAAGAGCCTGAGATGGAGGTTGATGTC
AACAATTGTTTTTTTGTAACAGTGACCAGAAAATTTTGATCTTTGGTCTTTTTACGAATT
TTTGTAACCAGGTTGAATAATTTATTAAAATTATGGTTTGATCATACAATTTTTTTAAAA
AAACTTACAGATTCTGTTTGAGCATTCTACAACCATAATACAAAGTTTTCTATTAACTAT
AACTATATGGTTTATCTACTGCAATG

Last Updated on 06.12.2018 16:11