Sequences from confirmation amplicon


Confirmation sequences for line 855C01 and hit At3g18130 are shown.
Line ID 855C01
Locus At3g18130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6211500
Primers used for PCR HM63 - 8474, show primer details
   
Sequence Name 01-K101221w-0022-855-C01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K101221w-0022-855-C01C-8409
TCGGACATGAGCCTTTACATTGAATATATCCTGGAAATCCCACCACCTAATTCAATTGGT
TACCAGTTCCAACACCACCTTCATTCTTCTCTGCCTCAGACTTGAGATCAACCTTCAAGT
CCTCAACAACAGACTTGCTCTCAAGATCCCAAATCCTAATGCTATTCTCAGTAGCAGCAC
ACAACCAGTATCTGTTAGGACTGAAGCAAAGCGAGTGAATAATCGAACCCGCCTCAAGCG
AGTAAAGCTTCTTTCCTTCAGCCAAATCCCACAACAAGATAACACCATCTTTCCCACCAC
TGGCGCATAGCGAACCATCAGGCGAGACAGCAACAGTGTTGAGGTAACCAGAGTGACCAA
CAAGAGAGTTCCTCAGCTTACAGTTCTGGAGATTCCAAACTTTCACAGTTTTATCCCAAG
AAGCAGATACAATAGTTGGTACAAGAGTATTAGGACTAAACCTAACACAACTAACCCATT
CCTTGTGACCATCACCTTCAGAGATGGTATACTTACACTCACCAAGTGTGTTCCAAAGCT
TAATCGTACGATCACGAGAAGCAGACACGATCTGCCGGTTATCAGTAGAGAATGCAAC
   
Sequence Name 65-K101228-0022-855-C01C-HM63
GenBank Accession LN511352 [GenBank]
Sequencing primer gene specific primer, HM63: 5'-TGTTGCATTCTCTACTGATAACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K101228-0022-855-C01C-HM63
CGATAAGCTTTGGAACACACTTGGTGAGTGTAAGTATACCATCTCTGAAGGTGATGGTCA
CAAGGAATGGGTTAGTTGTGTTAGGTTTAGTCCTAATACTCTTGTACCAACTATTGTATC
TGCTTCTTGGGATAAAACTGTGAAAGTTTGGAATCTCCAGAACTGTAAGCTGAGGAACTC
TCTTGTTGGTCACTCTGGTTACCTCAACACTGTTGCTGTCTCGCCTGATGGTTCGCTATG
CGCCAGTGGTGGGAAAGATGGTGTTATCTTGTTGTGGGATTTGGCTGAAGGAAAGAAGCT
TTACTCGCTTGAGGCGGGTTCGATTATTCACTCGCTTTGCTTCAGTCCTAACAGATACTG
GTTGTGTGCTGCTACTGAGAATAGCATTAGGATTTGGGATCTTGAGAGCAAGTCTGTTGT
TGAGGACTTGAAGGTTGATCTCAAGTCTGAGGCAGAGAAGAATGAAGGTGGTGTTGGAAC
TGGTAACCAATTGAATTAGGTGGTGGTGATTTCCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11