Sequences from confirmation amplicon


Confirmation sequences for line 856A11 and hit At1g53050 are shown.
Line ID 856A11
Locus At1g53050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19775124
Primers used for PCR 61zf - 8474, show primer details
   
Sequence Name 40-K125004-0022-856-A11C-61zf
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 61zf: 5'-TCATACAGGTTAAGTGCTACCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K125004-0022-856-A11C-61zf
TCGTTATCATAGTGCGTGGCCCAGGCGTGTGGATCCTGCTTTGGTACCGTCCACCTGCGC
TTTTGCTCGGAGCAACTCGCTATGGAGCAGCAGTTGATTTGTGGAGTGCAGGTTGCATTC
TTGCTGAACTATATGCAGGCAAGCCTATTATGCCTGGTAGAACCGAGGTAGATTTACAGT
TTTTGTTTTCTCTTTTAGTGTTTGCTCTGATTTATGTTAGACTAGTTTGGTTTCGTAAGG
TGACTCTGGTAATGCTTATGGTGTTACAGGTGGAACAGTTGCACAAGATTTTCAAGCTAT
GTGGCTCGCCTACCGAGGACTATTGGGTAAAATCAAGGTTGCCTCATGCAACAATTTTCA
AGCCTACTCAACCATATAAACGCTTAGTGGGTGAAACATTCAAGGAGTTTCCTCAGCCAG
CTTTAGCCCTTCTTGAAACTCTCCTTTCAGTCAATCCTGACGATCGTGGAACTGCCACTG
CAGCTCTCAAGAGTGAGGTACTGGCATTACTCAGGCAGTAAAAGCTGTGTGTTCTTATGC
GTCTGTATTTCCTGGTAATCGCTTTTGTAACATCAAATTATCATTCTTTTATTTTCAGTT
CTTCTCCACCCGACCTCTTCCATGTGATCCTTCAAGCTTGCCTAAATATCCTCCCAGCAA
AGAGCTCGATGCAAGAATGCGGGATGAGGAAAGTAG
   
Sequence Name 16-K125003-0022-856-A11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K125003-0022-856-A11C-8409
TTGAATTATCCTTTGCGGAAGCAGATCTACGTGATAGAGGACCAGAATGTGAAGCTCTCT
TGATGAGGAATTATATTGCCCTGAGATTCTCTGTTTGGTTCAAATGCTTGTGATGACGGC
CTTGGTGGATCGATTGGGAAACCGGATGCAACTTCTTCAGGATGTGGATTAAACTTCTCG
CTTCGACTTCTATTAGTAGACTGGCTCTGCCTTTTCTGCAATGACATAAATGCGAGAGGG
TCAAAAACGTTGAATAAAAGCAAAAAGCTTTAACATCGAAAGAAATAGATTTTTTAAGAT
TTTTTAACCTGCATTGATGCAACCAGCTCTGCATTTGCGTCAGGGGCTGGGATGGCTCGA
GATTCCTTAGTTCCTCTTCTTTCTTGGTGTCTTTGGTCCCTGTTTCCTCCAACTTGTCTA
CAAAAACAGGAACTAGATTAAAGCATTTCTATAAATATTTCTTTTTGCGTTAATGATACT
ATGCTATCGTAAGAGCATTGCAGGATAAAAGTCACATCAGACTAGAAAATCTGGATGGCA
TACCTTCTACTTTCCTCATCCCGCATTCTTGCATCGAGCTCTTTGCTGGGAGGATATTTA
GGCAAGCTTGAAGGATCACATGGAAGAGGTCTGGTGGAGAAGAACTGAAAATAAAAGAAT
GATAATTTGATGTTACAAAAGCGATTACCAGGAAATACAGACGCATAAGAACACACAGCT
TTTACTGCCTGAGTAATGCCAGTACCTCACTCTTGAGAGCTGCAGTGGCAGTTCCACGAT
CGTCAGGATTGACTGAAAGGAGAGTTTCAAGAAGGGCTAAAGCTGGCTGAGGAAACTCTT
GAATGTTTCACCCACTAAGCGTTTATATGGTTGAGTAGGCTTGAAAATTGTTGCATGAGG
CAACC
Primers used for PCR 12zg - 8474, show primer details
   
Sequence Name 24-K125509-0022-856-A11C-12zg
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 12zg: 5'-GATACCAATAACCGGCCTTCTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K125509-0022-856-A11C-12zg
CTCAACCGCAGAGAGATGGTTCTTGGGATTACCGTTGCTAAACCGGTTTCACAAGACTGA
GATAGCCTAAACCAGAAGCGGAGTCGTTTCTATCGTTGGGGACGATGATAAAGGCGAGGC
CATGACCGGGATTAGGATTTTTCGGGCGAGGACTAATGACGAAATAGAAAGAAGTTTTGA
AAGAGTAGACAGAGGAAGAAGTAGAACTAGGCTTAAAAGGGACTGGATTGATGAAAATGG
CTCGACCATGAGAGAATGGGACATTGTCACGGCTCAATCCAATTGCTCCTGACTTGCTCA
TACCATCAGGCTCTGAAGCGTACTCGGCGTCTCCATGGAATGTCATCAGATCAGTTCCAT
TTCCGAAGTAAATGAAGTCGAAACTGTAAGCAGAACACGTTATGGCGTGAGAGCAAAACA
GAGCAAAAAACAGAGGAATGAAACAGAGTGTTTTGATCTGCAATTCTGTTTATTTTTTTC
TTGGATATGGAAAGTATAAGAGGATAACAAGATTTATATAGTGAAAGAGAGCCAACAAAG
TTTGACTCGACAAGATCATTTATTTTTCACAAGTAAAGATCTCGTAACACCTTTCTTGGT
AAATTTCATAAAAAGTCACAAATTAAAAAGTTCAACTTTTGGAACATTTTCTAAATTATG
ATATATAACTCTGTTTCTACAGCTTTCTTATAATAAAATAATATCACATTACAGGGAAGC
TAAAACGTGAAAAGATTTGATTCTATGATGATATATAATCTTTGAATCATAAAATACAAT
TATTTTTCACTCAAAATACGCCGTGGTAAAAGGT
   
Sequence Name 11-K125508-0022-856-A11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K125508-0022-856-A11C-8409
ACAGAGACTTGCAGAGGAATGACTCGGCTTCCAGGTTCATTCAAGGAAACGTCTGAAGAA
GCAAACCAGGAAGAGAATGGTCGAAGCAACAAGAAAGACCCTATTCTCGTAAGTTCCCTA
AACGTCCCTCCTTTGCTAATTTTAGAAGACTAGCAATGTTAATATTCTGAGTTGTATGGT
TTGGTTTGCAGTTGGGTTATGGATCAAAAGGGCACAAGATTCATTACTCAGGACCGTTAG
TGGTTCCATCAGGAAACATGGATCAAGTGTTGAAAGACCATGACCGACATATCCAAGAAG
CTGTGAGAAGAGCACGAATCGATAAGGCTCGAGTCAAGAAACTTCAAGCTGACGAAGCTT
CGAGCCAACAAGTCGCAACCAATCATCCCTCATCTGTTTCTAGCCGTTGATCCATCAGTG
AGGCAAAAGTAAATGATCAAACCGAACCTCATTTGCCTGAAACCTTTTAACACGGCGTAT
TTTGAGTGAAAAATAATTGTATTTTATTGATTCAAAGATTATATATCATCATAGAATCAA
ATCTTTTCACGTTTTAGCTTCCCTGTAATGTGATATTATTTTATTATAAGAAAGCTGTAG
AAACAGAGTTATATATCATAATTTAGAAAATGTTCCAAAAGTTGAACTTTTTAATTTGTG
ACTTTTTATGAAATTTACCAAGAAAGGTGTTACGAGATCTTTACTTGTGAAAAATAAATG
ATCTTGTCGAGTCAAACTTTGTTGGCTCTCTTTCACTATATAAATCTTGTTATCCTCTTA
TACTTTCCATATCCAAGAAAAAAATAAACAGAATTGCAGATCNAAACACTCTGTTTCATT
CCTCTGTTTTTTGCTCTGTTTTGCTCTCACGCCATAACGTGTTCTGCTTACAGTTTCGAC
TTCATTTACTTCGGAAATGGAACTGATCTGATGACATTCCATG

Last Updated on 06.12.2018 16:11