Sequences from confirmation amplicon


Confirmation sequences for line 856E08 and hit At1g47730 are shown.
Line ID 856E08
Locus At1g47730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17564986
Primers used for PCR 35oc - 8474, show primer details
   
Sequence Name 46-K114909-0022-856-E08C-35oc
GenBank Accession LN505950 [GenBank]
Sequencing primer gene specific primer, 35oc: 5'-AGTCGATTTCCCTCCAAGAAGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K114909-0022-856-E08C-35oc
CGGTCTAGAAGTTTAGGAAAAGGAAGTTTAACACCTATAAGCATTTTTTCCTTTTCCAAT
TTAGTGGAAAAGGAAAAAGTTGAGACATATAAGCATAAATCGTGGAAAGGAAGTACCGAC
TTAGGGTTTTCTCCAAGATGGAGCAAAGAGAAGAGAAGACCGAAAATATCCAGAGAAAAA
GAAGCAGAGGAAAATCTTCATCATCATCCTTACCTCTCGATCTAACCTCGGAGATATTCT
CAAGGCTGCCTGCGAAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTC
   
Sequence Name 22-K114908-0022-856-E08C-8409
GenBank Accession LN496231 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114908-0022-856-E08C-8409
GAAGATTTTCCTCTGCTTCTTTTTCTCTGGATATTTTCGGTCTTCTCTTCTCTTTGCTCC
ATCTTGGAGAAAACCCTAAGTCGGTACTTCCTTTCCACGATTTATGCTTATATGTCTCAA
CTTTTTCCTTTTCCACTAAATTGGAAAAGGAAAAAATGCTTATAGGTGTTAAACTTCCAT
TTCCTAAACATCTAGACCGAGATAAAAAAAAATAGGCTCTATCTGACGTGATGTCAATAC
TTCTTGGA

Last Updated on 06.12.2018 16:11