Sequences from confirmation amplicon


Confirmation sequences for line 856G10 and hit At3g01770 are shown.
Line ID 856G10
Locus At3g01770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:276002
Primers used for PCR 65sm - 8474, show primer details
   
Sequence Name 56-K116569-0022-856-G10C-65sm
GenBank Accession LN509212 [GenBank]
Sequencing primer gene specific primer, 65sm: 5'-AATCGATTTGCGGATATAATCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K116569-0022-856-G10C-65sm
TGTGTTTGTGGAGGTGTGAGCTGTTATATCTGGTTAGCCCTCCCTCTTTGACTTTCAAAA
TTATATTTGTGAGATCAGAATGAGAAACGAGACCCAGAGACACTGCAACGTGAGAAGGAA
GAACTGGAATTGCAAAAGAAGAAAGGTATTCTTGATTTGCTAGTTTCGATTTTTTGATTG
ATGATATATACCAAGTAGTATATACGTACATAAACTTGTCCTCTTATTGTTTTGTGCACC
AGAAAAAGCACGGTTACAAGCAGAAGCCAAGGAAGCTGAAGAAGCTCGTAGAAAAAAGCA
ACTGAAAAAGAAAAACTACATGTCCGGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCAC
   
Sequence Name 27-K116568-0022-856-G10C-8409
GenBank Accession LN498557 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K116568-0022-856-G10C-8409
CATTTCATTGAATATATCCCGGACATGTAGTTTTTCTTTTTCAGTTGCTTTTTTCTACGA
GCTTCTTCAGCTTCCTTGGCTTCTGCTTGTAACCGTGCTTTTTCTGGTGCACAAAACAAT
AAGAGGACAAGTTTATGTACGTATATACTACTTGGTATATATCATCAATCAAAAAATCGA
AACTAGCAAATCAAGAATACCTTTCTTCTTTTGCAATTCCAGTTCTTCCTTCTCACGTTG
CAGTGTCTCTGGGTCTCGTTTCTCATTCTGATCTCACAAATATAATTTTGAAAGTCAAAG
AGGGAGGGCTAACCAGATATAACAGCTCACACCTCCACAAACACAGCTAACCTGATTAAG
AGTTATCTCTTGAGCCTTCAAGATTATATCCGCAAATCGATT

Last Updated on 06.12.2018 16:11