Sequences from confirmation amplicon


Confirmation sequences for line 857A04 and hit At4g11260 are shown.
Line ID 857A04
Locus At4g11260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6851892
Primers used for PCR MC14 - 8474, show primer details
   
Sequence Name 34-K035351-022-857-A04C-8409
GenBank Accession LN501322 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K035351-022-857-A04C-8409
TGAGCCTTTACAATTGAATATATTCAATATGAAACGCATAATGAGAAGTTAGCGTTTGTG
GAACCTGTTTCTTCACTTCAGCTTCCAACTTGTCCCAGTCTTTTGCTGGCTTAGAAGATG
GGTACACTGGTCTCTGCGACAGCGCTGCATATGCACATAAAATCAAATGAGAAGAGAGAG
AGACCACTTTGGGTTCAGTTTGTTTAAGGTTTTACGATTGTGGTTCAGAATAAAATAGTA
AACGTAAGCTGGTATCTTCTGAGACAAACCTGATGAGACATTGGGTTTGGGCAAAACACT
TTGCCCTTTACCATATTCAAGGGAGGCCCAGGTGATTATCTCTGCTTTCGCAAGACGGAT
CTCAACTTTGGTCGACAATACTTCAAATCTGCCTTCCTCTGGT
   
Sequence Name 82-K035351-022-857-A04C-MC14
GenBank Accession LN514143 [GenBank]
Sequencing primer gene specific primer, MC14: 5'-TACCAGAGAAGTGCAGATTTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K035351-022-857-A04C-MC14
CGAAGCAGAGATATCACCTCGGGCCTCCCTTGAATATGGTAAAGGGCAAAGTGTTTTGCC
CAAACCCAATGTCTCATCAGGTTTGTCTCAGAAGATACCAGCTTACGTTTACTATTTTAT
TCTGAACCACAATCGTAAAACCTTAAACAAACTGAACCCAAAGTGGTCTCTCTCTCTTCT
CATTTGATTTTATGTGCATATGCAGCGCTGTCGCAGAGACCAGTGTACCCATCTTCTAAG
CCAGCAAAAGACTGGGACAAGTTGGAAGCTGAAGTGAAGAAACAGGTTCCACAAACGCTA
ACTTCTCATTATGCGTTTCATATTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11