Sequences from confirmation amplicon


Confirmation sequences for line 857E04 and hit At1g75080 are shown.
Line ID 857E04
Locus At1g75080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28185812
Primers used for PCR JL15 - 8474, show primer details
   
Sequence Name 58-K032524-022-857-E04C-JL15
GenBank Accession LN509613 [GenBank]
Sequencing primer gene specific primer, JL15: 5'-AACCAATCAAACATCAATCAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K032524-022-857-E04C-JL15
ATGCATGACTTTGGACCAGTATCATACATAAAAGATTAAGACTTTAGCATACCAATAATA
ACTCTAAAATTAGAAAGAAAACCCATAAATTCACTTTGAGAAATCAGATCCATCCATAGT
TTCTGATGATTCAAATTCACTTTGAGAAAACCCTCAAATTCTCAGATTCAAACCCATTTA
ACCACAATTGAAATCAACGAATTCAGATTCAAACATCAAAAACATCAATAAAACAAGCTC
AGATCTGGAAAAAATGGAGAAAGTCTTCACCTTGCGATAAGTAGTACCATCTTCTTCAAC
AACCCAACCAGCTTCAACACAAAGAGCTTTAAGGACTTCATTATTATCACAATGTTTAGG
CAAATTATAATCACCTTGAGCTCTAAGCCCAGTGTATATCTTCGCAGCTACAGCTCTTCT
CCGTCTTTCTCTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 58-K032524-022-857-E04C-8409
GenBank Accession LN509612 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K032524-022-857-E04C-8409
CATTTACAATTGAATATATCCTGGAGAGAAAGACGGAGAAGAGCTGTAGCTGCGAAGATA
TACANCTGGGCTTAGAGCTCAAGGTGATTATAATTTGCCTAAACATTGTGATAATAATGA
AGTCCTTAAAGCTCTTTGTGTTGAAGCTGGTTGGGTTGTTGAAGAAGATGGTACTACTTA
TCGCAAGGTGAAGACTTTCTCCATTTTTTCCAGATCTGAGCTTGTTTTATTGATGTTTTT
GATGTTTGAATCTGAATTCGTTGATTTCAATTGTGGTTAAATGGGTTTGAATCTGAGAAT
TTGAGGGTTTTCTCAAAGTGAATTTGAATCATCAGAAACTATGGATGGATCTGATTTCTC
AAAGTGAATTTATGGGTTTTCTTTCTAATTTTAGAGTTATTATTGGTATGCTAAAGTCTT
AATCTTTTATGTATGATACTTGGTCCAAAGTCATTGCATTGTGTTTCTTTTGCTTACCTG
TGATTGATTGATGTTGGATTGGT

Last Updated on 06.12.2018 16:11