Sequences from confirmation amplicon


Confirmation sequences for line 857F12 and hit At5g25030 are shown.
Line ID 857F12
Locus At5g25030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8620678
Primers used for PCR 39sf - 8474, show primer details
   
Sequence Name 56-K116491-0022-857-F12C-39sf
GenBank Accession LN509210 [GenBank]
Sequencing primer gene specific primer, 39sf: 5'-ACTAATGCATCCCTGTGATGTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K116491-0022-857-F12C-39sf
GACTTAACATGCAACTGATCTGAGATGGATGTTCAGGAATCTAAAAGGTTAAGTCGATAT
AGCAATGAGCAAAAAATACTCGTGGTCGGAGAAGGAGAGTTTTCATTTTCTTTGTCTTTA
GCAAAAGCCTTGGGTTCTGCCACCAACATCACTGCAATATCGCTTGATATTCGAGGTAGT
AATATGTCTAAAATTATCAATCATTTTTTTTTCAATTTTGTTATTGTCGTTATCCGATAT
AAAGAACAATAGCTTATCAAGTTTGGCGATTATGTTTTGTATGGTAGAGGATCTAGGGCG
TAATTACAACAATGGAAAAGGGAACGTGGAAGAGTTGGTCAATATGAGTATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATAACCGCACG
GTTATT
   
Sequence Name 24-K116490-0022-857-F12C-8409
GenBank Accession LN497144 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K116490-0022-857-F12C-8409
ATTTACATTGAATAACTCATATTGACCAACTCTTCCACGTTCCCTTTTCCATTGTTGTAA
TTACGCCCTAGATCCTCTACCATACAAAACATAATCGCCAAACTTGATAAGCTATTGTTC
TTTATATCGGATAACGACAATAACAAAATTGAAAAAAAAATGATTGATAATTTTAGACAT
ATTACTACCTCGAATATCAAGCGATATTGCAGTGATGTTGGTGGCAGAACCCAAGGCTTT
TGCTAAAGACAAAGAAAATGAAAACTCTCCTTCTCCGACCACGAGTATTTTTTGCTCATT
GCTATATCGACTTAACCTTTTAGATTCCTGAACATCCATCTCAGATCAGTTGCATGTTTA
AGTCAATTCACACCATGTCCTTTTATACACATGTACATACACATCACAGGGAGGCATTA

Last Updated on 06.12.2018 16:11