Sequences from confirmation amplicon


Confirmation sequences for line 858B06 and hit At4g17900 are shown.
Line ID 858B06
Locus At4g17900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9947691
Primers used for PCR MA76 - 8474, show primer details
   
Sequence Name 30-K102512-0022-858-B06C-8409
GenBank Accession LN499664 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K102512-0022-858-B06C-8409
ATTTACATTGAAGATCGGTGAGGAATTCCCTTTCTTCGCTTGACGATTCTGCAGTTACTA
GATGTAGTAAGTGGAGGTGTTGATGGGCTGAAACTCTGGAGATTTGTAATGTTCTTCCCA
ATTGCAATGCTGCTACTTGAATCCTCTGTTTCCATTAGCAGGTTCTCCCTTCCCTTTTCA
AAGCCTCTTGATGTTCCAGCAATCTTGATTTCATTTCCCAAAAACATAGAGAAAGTAAGT
ATCAGAAAAGAGTTTACTGAAATGCAAGGAGAAGTTAGGTGAAACTGTAAAACATATATC
AGATTTCAGGATGACCCATTTCAAAAGTTTTGATCTTTTCTTCATGCGGTTAAAAAGGAA
TTGAATTTACCAAAAATGCAAGCAAATGTTAGGTGAAGCTGTGTGAGACCTGATTTGCTA
TATGAAACGTGTAAAGAGGGAATCAAAAGTGTCAACAAAATACCA
   
Sequence Name 49-K102513-0022-858-B06C-MA76
GenBank Accession LN506795 [GenBank]
Sequencing primer gene specific primer, MA76: 5'-TTGGTATTTGGTTGACACTTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K102513-0022-858-B06C-MA76
ATCAGGTCTCACACAGCTTCCCTAACATTTGCTTGCATTTTTGGTAAATTCAATTCCTTT
TTAACCGCATGAAGAAAAGATCAAAACTTTTGAAATGGGTCATCCTGAAATCTGATATAT
GTTTTACAGTTTCACCTAACTTCTCCTTGCATTTCAGTAAACTCTTTTCTGATACTTACT
TTCTCTATGTTTTTGGGAAATGAAATCAAGATTGCTGGAACATCAAGAGGCTTTGAAAAG
GGAAGGGAGAACCTGCTAATGGAAACAGAGGATTCAAGTAGCAGCATTGCAATTGGGAAG
AACATTACAAATCTCCAGAGTTTCAGCCCATCAACACCTCCACTTACTACATCTAGTAAC
TGCAGAATCGTCAAGCGAAGAAAGGGAATTCCTCACCGATCTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11