Sequences from confirmation amplicon


Confirmation sequences for line 858G02 and hit At3g28007 are shown.
Line ID 858G02
Locus At3g28007 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10408703
Primers used for PCR 29ci - 8474, show primer details
   
Sequence Name 55-K107936-0022-858-G02C-29ci
GenBank Accession LN508835 [GenBank]
Sequencing primer gene specific primer, 29ci: 5'-CTTCATCTTCATCATCTTTTGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107936-0022-858-G02C-29ci
AGTTGACTGCTCCTGATCCGTTCCAGTCCATTTCCAATCTATGAAAAAAATAACTAAGTG
AAACTACTAATAGAAGAAGAATAGTATATAGTAACATATATCATTTAATAAACTTACCAA
AATGAAAAGGTCGAACTTAATAAGTGCATAAATAACCCAAACGACACCATTGAGGAAATT
GGCAAGTGAGAGAGAGAATGGCATGTACTTCACACTTTTGGTCTTGATCACCTTACTCTG
TTTCAATCACAACAAAAATATCATGTTTTTAAATTATTGAAAATGAGAGAAAATACTAAA
TATTTAAATAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 39-K107935-0022-858-G02C-8409
GenBank Accession LN503378 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K107935-0022-858-G02C-8409
ATTTACATTGAATATTATTTAAATATTTAGTATTTTCTCTCATTTTCAATAATTTAAAAA
CATGATATTTTTGTTGTGATTGAAACAGAGTAAGGTGATCAAGACCAAAAGTGTGAAGTA
CATGCCATTCTCTCTCTCACTTGCCAATTTCCTCAATGGTGTCGTTTGGGTTATTTATGC
ACTTATTAAGTTCGACCTTTTCATTTTGGTAAGTTTATTAAATGATATATGTTACTATAT
ACTATTCTTCTTCTATTAGTAGTTTCACTTAGTTATTTTTTTCATAGATTGGAAATGGAC
TTGGAACGGTATCAGGAGCAGTACAACTTATACTCTATGCTTGCTATTACAAGACAACAC
CAAAAGATGATGAAGATGAAG
Primers used for PCR 70wl - 8474, show primer details
   
Sequence Name 73-K118446-0022-858-G02C-70wl
GenBank Accession LN512974 [GenBank]
Sequencing primer gene specific primer, 70wl: 5'-GAGGAGTACAAAGCTGACCCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K118446-0022-858-G02C-70wl
TGCGCATAGGGTCTTTATGGCTTACCAATGGTTCACCAGATAGTCTCCTTGTGATCACCA
TAAATGGTACCGGTTTAGCCATTGAGCTGGTGTATCTCGCTATCTTCTTCTTCTTTTCTC
CAACTAGTCGCAAGGTTTAGTAATTTCCATTCATATCTCAATTCTTTTCACTTAATTTTA
TTAAATGTTTTTTCCAATCATTATCTAAACCTAAAATCGCTATTAGGTTTGGCTTAAATT
GAAAGCTAAAACATTATAGTAATAAGAAGTGGTAGACTATGATTGATTCTGTTTTAGGAT
AGTGATAGGCAAAACAAAATGTTTGTAGATTTTTTTTTTTTTTTCGAAAATTATTTAAAC
CAAATGGGTTCAGGGGGCAGGGAAAAGGGGGGCTATGGTAAAAAGAAAAAATGGTTTTTG
TAGAAATATAACCCCCTTGCCCTTTGTTTTTGTTCCACACACATAACCAAAAATTTTCTT
TTGTGGAAACCTTTGGGGCCATTTTTGTAAGCCCCAGGAATATGGCCCCCCCCCCCACCG
GGGTAAAATATTATTTAAAAATTTCCGGAAAATATTATTTATGGTTTCCCCGAAAATCCA
ATTGAAAAGGGCTTCATGCCCGGAAAATCTCCTGGGATCACCAAGGAGTATAAGGGTCAA
TAGGGAAAAAAAAAAAAAGTATTTCCCAATTTTTTTTCATTTCAAAAAGGTAAATTT
   
Sequence Name 33-K118445-0022-858-G02C-8409
GenBank Accession LN501186 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118445-0022-858-G02C-8409
TCATTGAAATCCGGGAAAACATAAATAATATTTCCCGGATATTTATAAATAATATTTTAC
CATGATGGTGAGAGGAGCAATATACATGAGACTAACAAAAATGACACAAAAGATTCCAAC
AAAAGAAGATCTCTGGTTATGTGTGTGGAACAATAGCAATGTGCATGTGGCTACTATTCC
TACAAACACCATCTCTCCTATTAACCATAGCCCCACTTTCACCTGCACCATGAACCAATT
TGATTAAAATAATTTCAGAAAAAAAAAAAAAATTCTCCAAACTTTTTGTTTTGCCTATCA
TTTTCCAAAAACAAAATCAATCAAAGCCCCCCCCTTCTTATAACTATAAGGTTTAACCTT
TCATTTTAACCCAAACCTAAAAGCGTTTTTGGGTTTAAATAAGGATGGGAAAAAACTTTT
AAAAAATTTAAGTGAAAAAAATTGAAATTTGAAGGGAATTTACTAACCCTTGCAACTATT
TGGAAAAAAAAAAAAAAAAATGGCGAGATCCCCCACCTCAAGGGCAAACCCGGTCCCTTT
TTTGGGGATCACAAGGAAACTATTTGGTTAAACCTTTGGTAACCCATAAAAAACCCATAG
GGCCCATTTTAAAACCGGGGCAAATTAGGGGT

Last Updated on 06.12.2018 16:11