Sequences from confirmation amplicon


Confirmation sequences for line 859D12 and hit At2g42680 are shown.
Line ID 859D12
Locus At2g42680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17775941
Primers used for PCR 83wb - 8474, show primer details
   
Sequence Name 33-K118262-0022-859-D12C-83wb
GenBank Accession LN501178 [GenBank]
Sequencing primer gene specific primer, 83wb: 5'-TTCGTTTTTGATTGAACTAGCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118262-0022-859-D12C-83wb
AGATCTTTTAGGGTTTATGTTCGTTGTCAATTGAAATTTCCGGTGTATCTGTATTGCTGA
TTTAGTTGTTGTTCATATTAGGTCGATAATTTCTGCTGGTTTTATTTTGGTTTTTATGTG
CAATTTCTAGATTTGGCTTCCATCTAACATTGATTTTGTGTGTGTGTTGGGTTTAGTCAA
TGCTGGAACCAACAAGGCGGCATCAAGCGGCACATCTCTGAACACAAAAATGCTTGATGA
TGACACTGAGAACCTTACTCGTAAGTCTTATCTTTAATCATTTGGTTTATTAATGGTGAA
TTGATTTCCCTAGTGTCTAGAATATGGTAGTGTCAGGCTCTGTATCTAGGCTAATGTGTT
CATTTCTTGTTTCAAAGTGTTCAACGTATTGGAAAACTGTTCTTATATGAGTAGAGTCTG
TCTATCTTTCTGCCTGTGAGTCTTTGATGGTTATAGTGAGAGGAACTTTTTATTGATTGA
TGTGTATTGGATGCTGAAGCTTCATGTTTTGAATTATGCAATTGTGAATTCAGATGAACG
TGTGCCTACTGAGCTAAAGAAAGCCATTATGCAAGCCAGGACAGACAAGAAGCTAACCCA
GTCCCAACTTGCTCAAGTATATTATCCTTCTCTCTATATGTCCCTTATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 13-K118261-0022-859-D12C-8409
GenBank Accession LN492248 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118261-0022-859-D12C-8409
ATTGAATAAGGGACATATAGAGAGAAGGATAATATACTTGAGCAAGTTGGGACTGGGTTA
GCTTCTTGTCTGTCCTGGCTTGCATAATGGCTTTCTTTAGCTCAGTAGGCACACGTTCAT
CTGAATTCACAATTGCATAATTCAAAACATGAAGCTTCAGCATCCAATACACATCAATCA
ATAAAAAGTTCCTCTCACTATAACCATCAAAGACTCACAGGCAGAAAGATAGACAGACTC
TACTCATATAAGAACAGTTTTCCAATACGTTGAACACTTTGAAACAAGAAATGAACACAT
TAGCCTAGATACAGAGCCTGACACTACCATATTCTAGACACTAGGGAAATCAATTCACCA
TTAATAAACCAAATGATTAAAGATAAGACTTACGAGTAAGGTTCTCAGTGTCATCATCAA
GCATTTTTGTGTTCAGAGATGTGCCGCTTGATGCCGCCTTGTTGGTTCCAGCATTGACTA
AACCCAACACACACACAAAATCAATGTTAGATGGAAGCCAAATCTAGAAATTGCACATAA
AAACCAAAATAAAACCAGCAGAAATTATCGACCTAATATGAACAACAACTAAATCAGCAA
TACAGATACACCGGAAATTTCAATTGACAACGAACATAAACCCTAAAAAGATCTCCTTAT
CATGAATCACAATAACCCCAAGGCATTGAAGGCTAGT

Last Updated on 06.12.2018 16:11