Sequences from confirmation amplicon


Confirmation sequences for line 859H06 and hit At1g69780 are shown.
Line ID 859H06
Locus At1g69780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26259755
Primers used for PCR KS35 - 8474, show primer details
   
Sequence Name 94-K033100-022-859-H06C-8409
GenBank Accession LN515274 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K033100-022-859-H06C-8409
CATTTACATTGAATATATCCAAGCTGAGGTAATTAATCTCATAAATTAACAAAAAAAATC
AATATGTGTTATTTTTTTTTGGGTTAATGATCAATAATTACAGTTATTTTCCATCTAAAG
GATGATTTTTTTCTTTTTAAAAAAGGTTAAAAATTATATTTCTGGTTTATAATTATTTGG
ATCAGGAGTTGCTTTCAGGTAGGGTTAAAAAACTGGACATGATTCATGACTTTTCAGACA
TCATTATCTCTTTTTTTCTTCACTCTTGTCTGGAAAGAGATCTGAAAACAATAGTTTCTT
TATGCTTATCACATTGTACAGTAACTCTGTTTATGTTTAAAATTTTGTCTTTAATTACGC
AGATAATGGGATTAAAAAACAGAGAACAAACAGAATCAATAAATCTAAACAAAGAAACTG
AAGGATCTTGCAGTAACAGAAGTGATAACAGTTCAGATAATCTCAGACTAGATATCTCAA
CTGCGCCGCCATCAAACGACAGTACATTAACCGGTGGCCACCCACCGCCACCACAGACAG
TTGGTCGACACTTCTTCCCACCGTCGCCAGCCACCGCAACGACA
   
Sequence Name 46-K033100-022-859-H06C-KS35
GenBank Accession LN505778 [GenBank]
Sequencing primer gene specific primer, KS35: 5'-ATTGTTGTAGTAGTTGTCGTTGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K033100-022-859-H06C-KS35
ACTGTCTGTGGTGGCGGTGGGTGGCCACCGGTTAATGTACTGTCGTTTGATGGCGGCGCA
GTTGAGATATCTAGTCTGAGATTATCTGAACTGTTATCACTTCTGTTACTGCAAGATCCT
TCAGTTTCTTTGTTTAGATTTATTGATTCTGTTTGTTCTCTGTTTTTTAATCCCATTATC
TGCGTAATTAAAGACAAAATTTTAAACATAAACAGAGTTACTGTACAATGTGATAAGCAT
AAAGAAACTATTGTTTTCAGATCTCTTTCCAGACAAGAGTGAAGAAAAAAAGAGATAATG
ATGTCTGAAAAGTCATGAATCATGTCCAGTTTTTTAACCCTACCTGAAAGCAACTCCTGA
TCCAAATAATTATAAACCAGAAATATAATTTTTAACCTTTTTTAAAAAGAAAAAAATCAT
CCTTTAGATGGAAAATAACTGTAATTATTGATCATTAACCCAAAAAAAAATAACACATAT
TGATTTTTTTTGTTAATTTATGAGATTAATTACCTCAGCTTGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACCAATTACGATCCGT

Last Updated on 06.12.2018 16:11