Sequences from confirmation amplicon


Confirmation sequences for line 860A04 and hit At1g58684 are shown.
Line ID 860A04
Locus At1g58684 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21770190
Primers used for PCR 08yj - 8474, show primer details
   
Sequence Name 18-K120096-0022-860-A04C-oriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K120096-0022-860-A04C-oriL
CATTGAATATATCCTGAGTCAACACTATGGTTAAGATAACACCAACAACATCAACACTAT
GGTTAAGATAATCAAGCAACATCATGCCAAGAGTTCAAAAAAGAAATCAAGAATATCAAT
CTATCACAAATTATGAAGCATCTACATCCATCAAATTAAGTACTTGAGATGAAAATCATC
AACAAAATCTATGATCATCTACAAATTGCAATCAGCTACATTTAGACGCTTCATTTATCA
CCAACTACTAAGCAAATGTGAACTAAATCCCTATTAAGGGATAATCAAATCATTAAAAGC
AGAGCTAAATCAAAGAACTCTACAGCTAAACATTATCAAATGCTTCAAAAAAGAAGGATC
AAAATCAAACCTTGACAAAGTTTCCAAGGGTTTTGGTAGATCCTCTTGAAGAAGTAAAGA
CATCATCAATACCAGCGAACTGAAGAACCTTCTTAGGAACCCTAGCAGCAACAATACCAG
AACCTCTCGGAGCAGGAACCATTCTCACAGTAACAGAACCACATTTACCAGTAACCTTAC
AAGGCACAGTGTGTGGCTTCCCAATCTTATTCCCCCAATAACCTCTCCTCACCGGAACCA
CTGATAGCTTAGCAAGAATAATAGCTCCTCTAATGGCAGTAGCAACTTCCTTAGAACACT
TGACACCCAAACCAACATGACCATTTCCATCACCAACAACAACAAAGGCCTTGAATCTAG
TCCTTTGACCAGCTCTGGTTTGTTTCTGAACAGGCATGATCTTCATAACCTCGTCTTTCA
ACGTAGGACCAACAAGATGATCTATGATTTGGTACTCCTTTACTGGGAGAGAATGAAGAT
AGATCTGCTCTAGCTTCGTTATTTTATTGTCAGCCACTAGACGACCTAGTTTGGTCACTG
GAACCCATTTCGTTTCTTCCGAGGCACGGCCTCCA
   
Sequence Name 37-K120093-0022-860-A04C-08yj
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 08yj: 5'-CGGTGACCGTGGTGACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K120093-0022-860-A04C-08yj
TCGTGGTCCAGAGGCCGTGGAAGACGTGGAGGCCGTGCCTCGGAAGAAACGAAATGGGTT
CCAGTGACCAAACTAGGTCGTCTAGTGGCTGACAATAAAATAACGAAGCTAGAGCAGATC
TATCTTCATTCTCTCCCAGTAAAGGAGTACCAAATCATAGATCATCTTGTTGGTCCTACG
TTGAAAGACGAGGTTATGAAGATCATGCCTGTTCAGAAACAAACCAGAGCTGGTCAAAGG
ACTAGATTCAAGGCCTTTGTTGTTGTTGGTGATGGAAATGGTCATGTTGGTTTGGGTGTC
AAGTGTTCTAAGGAAGTTGCTACTGCCATTAGAGGAGCTATTATTCTTGCTAAGCTATCA
GTGGTTCCGGTGAGGAGAGGTTATTGGGGGAATAAGATTGGGAAGCCACACACTGTGCCT
TGTAAGGTTACTGGTAAATGTGGTTCTGTTACTGTGAGAATGGTTCCTGCTCCGAGAGGT
TCTGGTATTGTTGCTGCTAGGGTTCCTAAGAAGGTTCTTCAGTTCGCTGGTATTGATGAT
GTCTTTACTTCTTCAAGAGGATCTACCAAAACCCTTGGAAACTTTGTCAAGGTTTGATTT
TGATCCTTCTTTTTTGAAGCATTTGATAATGTTTAGCTGTAGAGTTCTTTGATTTAGCTC
TGCTTTTAATGATTTGATTATCCCTTAATAGGGATTTAGTTCACATTTGCTTAGTAGTTG
GTGATAAATGAAGCGTCTAAATGTAGCTGATTGCAATTTGTAGATGATCATAGATTTTGT
TGATGAATTTCATCTTCAGTACTTAATTTGATGGATGTAGATGCTTCATAATTTGTGATA
GATTGATATTCTTGATTTCTTT
Primers used for PCR 83yh - 8474, show primer details
   
Sequence Name 17-K120096-0022-860-A04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K120096-0022-860-A04C-8409
ATTTACAATTGAATATATCCTGAGTCAACACTATGGTTAAGATAACACCAACAACATCAA
CACTATGGTTAAGATAATCAAGCAACATCATGCCAAGAGTTCAAAAAAGAAATCAAGAAT
ATCAATCTATCACAAATTATGAAGCATCTACATCCATCAAATTAAGTACTTGAGATGAAA
ATCATCAACAAAATCTATGATCATCTACAAATTGCAATCAGCTACATTTAGACGCTTCAT
TTATCACC
   
Sequence Name 36-K120093-0022-860-A04C-83yh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 83yh: 5'-TGGTGATAAATGAAGCGTCTAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K120093-0022-860-A04C-83yh
ATTTTGTTGATGATTTTCATCTCAAGTACTTAATTTGATGGATGTAGATGCTTCATAATT
TGTGATAGATTGATATTCTTGATTTCTTTTTTGAACTCTTGGCATGATGTTGCTTGATTA
TCTTAACCATAGTGTTGATGTTGTTGGTGTTATCTTAACCATAGTGTTGACTCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCATTATTA
Primers used for PCR 12yk - 8474, show primer details
   
Sequence Name 38-K120095-0022-860-A04C-12yk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 12yk: 5'-TGCACTCAATTTGCATAAAGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K120095-0022-860-A04C-12yk
TAAGCAAAGCAATTCCACTCAATTTGCATATCACGTACTATATTGGGTGTTATTGAATAA
GTTATGTTATTGGGCCGGGNCTTCTTGGTTTGTATCTCAACAAAACAATTATATCAACAT
TCGGCCCACAAGCCCATAATTCAGACTTTCGAAAACCCTAATCTTATGCTCACCTATATA
AACTAAAACTTGTGAATCCGCTAGGGTTTACTTCATCGAAAATGGCGGAAAGAGGAGGAG
AAAGCGGCGCAGAGCGTGGCGGTGACCGTGGTGACTTCGGGCGTGGATTCGGCGGTGGAC
GTGGAGGTGGCCGTGGCCGTGATCGTGGTCCAAGAGGCCGTGGAAGACGTGGAGGCCGTG
CCTCGGAAGAAACGAAATGGGTTCCAGTGACCAAACTAGGTCGTCTAGTGGCTGACAATA
AAATAACGAAGCTAGAGCAGATCTATCTTCATTCTCTCCCAGTAAAGGAGTACCAAATCA
TAGATCATCTTGTTGGTCCTACGTTGAAAGACGAGGTTATGAAGATCATGCCTGTTCAGA
AACAAACCAGAGCTGGTCAAAGGACTAGATTCAAGGCCTTTGTTGTTGTTGGTGATGGAA
ATGGTCATGTTGGTTTGGGTGTCAAGTGTTCTAAGGAAGTTGCTACTGCCATTAGAGGAG
CTATTATTCTTGCTAAGCTATCAGTGGTTCCGGTGAGGAGAGGTTATTGGGGGAATAAGA
TTGGGAAGCCACACACTGTGCCTTGTAAGGTTACTGGTAAATGTGGTTCTGTTACTGTGA
GAAGGGTCCTGCTCCGAGAGGTTCTGGTATTGTTGCTGCTAGGGTTCCTAAAAAGGTTCT
TCCGTTCGCTGGTATTGGATGAT
   
Sequence Name 19-K120094-0022-860-A04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K120094-0022-860-A04C-8409
CTTTACATTGAATATATCCTGAGTCAACACTATGGTTAAGATAACACCAACAACATCAAC
ACTATGGTTAAGATAATCAAGCAACATCATGCCAAGAGTTCAAAAAAGAAATCAAGAATA
TCAATCTATCACAAATTATGAAGCATCTACATCCATCAAATTAAGTACTTGAGATGAAAA
TCATCAACAAAATCTATGATCATCTACAAATTGCAATCAGCTACATTTAGACGCTTCATT
TATCACCAACTACTAAGCAAATGTGAACTAAATCCCTATTAAGGGATAATCAAATCATTA
AAAGCAGAGCTAAATCAAAGAACTCTACAGCTAAACATTATCAAATGCTTCAAAAAAGAA
GGATCAAAATCAAACCTTGACAAAGTTTCCAAGGGTTTTGGTAGATCCTCTTGAAGAAGT
AAAGACATCATCAATACCAGCGAACTGAAGAACCTTCTTAGGAACCCTAGCAGCAACAAT
ACCAGAACCTCTCGGAGCAGGAACCATTCTCACAGTAACAGAACCACATTTACCAGTAAC
CTTACAAGGCACAGTGTGTGGCTTCCCAATCTTATTCCCCCAATAACCTCTCCTCACCGG
AACCACTGATAGCTTAGCAAGAATAATAGCTCCTCTAATGGCAGTAGCAACTTCCTTAGA
ACACTTGACACCCAAACCAACATGACCATTTCCATCACCAACAACAACAAAGGCCTTGAA
TCTAGTCCTTTGACCAGCTCTGGTTTGTTTCTGAACAGGCATGATCTTCATAACCTCGTC
TTTCAACGTAGGACCAACAAGATGATCTATGATTTGGTACTCCTTTACTGGGAGAGAATG
AAGATAGATCTGCTCTAGCTTCGTTATTTTATTGTCAGCCACTAGACGACCTAGTTTGGT
CACTGGAAACCATTTCGTTTCTTCCGAGGCACGGCCTCAACGTCTTCC

Last Updated on 06.12.2018 16:11