Sequences from confirmation amplicon


Confirmation sequences for line 861E01 and hit At4g22070 are shown.
Line ID 861E01
Locus At4g22070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11693741
Primers used for PCR IS83 - 8474, show primer details
   
Sequence Name 93-K027643-022-861-E01C-8409
GenBank Accession LN515217 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K027643-022-861-E01C-8409
TCGCTTCCCGTATTCGCGGTCAGAAGATTCAGACCGATCTATATATATATTTACACAGTA
TTAATACATTAACCAGATTCAGTTTATCTGTGTAGTAATGTAAAAATAATAAAATTAACT
GTTTGCAAAATAATTGTTGCTAGTGTTATGAGCATTCACATCACATCTCACCAACCAAAG
AAATATATTTCAACACATATAAGAAATAAAATAAATCATTTAATATCGATAGCAAAAATT
AGGAGAAAATCAACTATACTTACATTGACATCTCTGGAACGATCGTTTTCTTCAACTCGT
GAACCCTCCATTTTGATGAGAACCTTATTGCCTTCGTCGTCGTCGTCGTTGATGTTCTCA
CGTGAAACCCTATCTCTCTTCTCTGAGAAGAATC
   
Sequence Name 94-K027643-022-861-E01C-IS83
GenBank Accession LN515269 [GenBank]
Sequencing primer gene specific primer, IS83: 5'-TTGATGAGGTTGACTTCTTCTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K027643-022-861-E01C-IS83
ATCAACGACGACGACGACGAAGGCAATAAGGTTCTCATCAAAATGGAGGGTTCACGAGTT
GAAGAAAACGATCGTTCCAGAGATGTCAATGTAAGTATAGTTGATTTTCTCCTAATTTTT
GCTATCGATATTAAATGATTTATTTTATTTCTTATATGTGTTGAAATATATTTCTTTGGT
TGGTGAGATGTGATGTGAATGCTCATAACACTAGCAACAATTATTTTGCAAACAGTTAAT
TTTATTATTTTTACATTACTACACAGATAAACTGAATCTGGTTAATGTATTAATACTGTG
TAAATATATATATAGATCGGTCTGAATCTTCTGACCGCGAATACGGGAAGCGATGAGTCA
ACGGTGGATGATGGACTATCATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11