Sequences from confirmation amplicon


Confirmation sequences for line 863C05 and hit At2g28400 are shown.
Line ID 863C05
Locus At2g28400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12149099
Primers used for PCR NU62 - 8474, show primer details
   
Sequence Name 38-K114664-0022-863-C05C-NU62
GenBank Accession LN503077 [GenBank]
Sequencing primer gene specific primer, NU62: 5'-GTACGTTAACCGGTAGAGAAGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K114664-0022-863-C05C-NU62
GGTTCGACCGGATGTGATTGTAAGGTCACTCCCTCTTAAACTTGAAGAGGAATCTGAACC
GGTATTGAAAAGATCCCATTCGTCTAGCTCGAAATCGGAAGTGGCGGTGACGTGTTGGTC
AGTGGTGAAGAAACGGTGGCTTGGCCGTGGATAGTAGCACTTGCTCGTCGCCATTTCAAG
AAGAAGAAGAAATGAAACTTTGTTTTTGGTTTTAAGCAAAGTTTTAAGATTCTTTTAGGC
TTTGGGTTTTGCTTCTTTATATATTGGTGAAGGTCAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 09-K114663-0022-863-C05C-8409
GenBank Accession LN490290 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114663-0022-863-C05C-8409
CTTCCCAATATATAAAGAAGCAAAACCCAAAGCCTAAAAGAATCCTAATAACTTTGCTTA
AAACCAAAAACAAAGTTTCATTTCTTCTTCTTCTTGAAATGGCGACGAGCAAGTGCTACT
ATCCACGGCCAAGCCACCGTTTCTTCACCACTGACCAACACGTCACCGCCACTTCCGATT
TCGAGCTAGACGAATGGGATCTTTTCAATACCGGTTCAGATTCCTCTTCAAGTTTCAGCT
TTAGTGACCTTACAATCACATCCGGTCGAACCGGAACTAACCGGCAAATTCACGGTGGTT
CTGACTCCGGTAAAGCTGCGTCTTCTCTACCGTTTAACGTA

Last Updated on 06.12.2018 16:11