Sequences from confirmation amplicon


Confirmation sequences for line 863E04 and hit At5g63140 are shown.
Line ID 863E04
Locus At5g63140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25329549
Primers used for PCR 26ey - 8474, show primer details
   
Sequence Name 90-K111271-0022-863-E04C-26ey
GenBank Accession LN515050 [GenBank]
Sequencing primer gene specific primer, 26ey: 5'-CATGACATTGTTGTTTTAACCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K111271-0022-863-E04C-26ey
CGCAAGCCTAATCCTCAGAAGGTATAGCTCCAGGACTAGCTTACTTCCACATTCCATTAC
CGGAGTTTTTGAGTTTTGACTCAAAGAACGCTACAAAAGGAGTGAGACAAGAAGGTACAT
CCGCGGCTTCTACAAACTCGGGTTTCTTCACAACATTGATAGCTAGAGGAGATGTGAAAT
CTGTGTTTGTGGGTCACGACCATGTCAACGACTTCTGCGGTGAACTTAAAGGTTTGAATC
TATGTTATGGTGGTGGATTTGGGTATCATGCTTATGGTAAAGCTGGATGGGAAAGAAGAG
CGAGAGTTGTGGTTGTTGATTTGAATAAGAAGAGAAAAGGAAAGTGGGGAGCTGTGAAAT
CGATTAAGACATGGAAGAGGCTTGATGATAACAAATTACCAATATTGATTCCATGTGTTG
TTGTAAATATTTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 75-K111270-0022-863-E04C-8409
GenBank Accession LN513262 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K111270-0022-863-E04C-8409
TTGTAATATTTACAACAACACATGGAATCAATATTGGTAATTTGTTATCATCAAGCCTCT
TCCATGTCTTAATCGATTTCACAGCTCCCCACTTTCCTTTTCTCTTCTTATTCAAATCAA
CAACCACAACTCTCGCTCTTCTTTCCCATCCAGCTTTACCATAAGCATGATACCCAAATC
CACCACCATAACATAGATTCAAACCTTTAAGTTCACCGCAGAAGTCGTTGACATGGTCGT
GACCCACAAACACAGATTTCACATCTCCTCTAGCTATCAATGTTGTGAAGAAACCCGAGT
TTGTAGAAGCCGCGGATGTACCTTCTTGTCTCACTCCTTTTGTAGCGTTCTTTGAGTCAA
AACTCAAAAACTCCGGTAATGGAATGTGGAAGTAAGCTAGTCCTGGAGCTATACCTTCTT
GAGGATTAGGCTTTGCGTTGTACTCCCTCTGCAATATCATCAAAACCAGGGTTAAAACAA
CAATGTCATG

Last Updated on 06.12.2018 16:11