Sequences from confirmation amplicon


Confirmation sequences for line 863E08 and hit At1g04430 are shown.
Line ID 863E08
Locus At1g04430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1199434
Primers used for PCR LD12 - 8474, show primer details
   
Sequence Name 12-K030753-022-863-E08C-8409
GenBank Accession LN491449 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K030753-022-863-E08C-8409
GCCATTTACAATTGAATATATCCTCGCCTATACAAGACAATATCACAAATCAATATGAAG
CCATTGCAAAACAATATCAAGACAATGTAACCAGGAACCAATCACAAATCAAAGCAAAAC
AAGAAAGCAAATATAGCACCTTGTATCCAGATGGTGGAGGAATCAAACAATTAAACCGTC
TTTCGGGAGGAGGACAATGCCGTTCATAATGTTCCATTAAAGAGAGATCAAGCTTCAACC
TCATTTGGTAGATGAAATTCCTATCTAAGCAAGGGATGATTTCCGAGTGCCGGTCATCAC
AAACCTTAACATTTAAACCATAAGTTAGCTAACATTATTAACATATCTCAACCAGTTACC
AGACCTGAGAATCTGATCAAACTTACAGGAAAACTTTTGGCGACAACAAGACTGTCTTCT
GCATTAGCCACAGAATCATCCTGTTTGGTATCACCATTATCATCATCTCCACTCAAATAA
GACGATCCGAGTTTTTCTC
   
Sequence Name 51-K030753-022-863-E08C-LD12
GenBank Accession LN507394 [GenBank]
Sequencing primer gene specific primer, LD12: 5'-TTGAGAAAACTCGGATCGTCTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K030753-022-863-E08C-LD12
ATGGTGATCCAACAGGATGATTCTGTGGCTAATGCAGAAGACAGTCTTGTTGTCGCCAAA
AGTTTTCCTGTAAGTTTGATCAGATTCTCAGGTCTGGTAACTGGTTGAGATATGTTAATA
ATGTTAGCTAACTTATGGTTTAAATGTTAAGGTTTGTGATGACCGGCACTCGGAAATCAT
CCCTTGCTTAGATAGGAATTTCATCTACCAAATGAGGTTGAAGCTTGATCTCTCTTTAAT
GGAACATTATGAACGGCATTGTCCTCCTCCCGAAAGACGGTTTAATTGTTTGATTCCTCC
ACCATCTGGATACAAGGTGCTATATTTGCTTTCTTGTTTTGCTTTGATTTGTGATTGGTT
CCTGGTTACATTGTCTTGATATTGTTTTGCAATGGCTTCATATTGATTTGTGATATTGTC
TTGTATAGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11