Sequences from confirmation amplicon


Confirmation sequences for line 864C02 and hit At3g02885 are shown.
Line ID 864C02
Locus At3g02885 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:638371
Primers used for PCR DB19 - 8474, show primer details
   
Sequence Name 41-K030143-022-864-C02C-8409
GenBank Accession LN503960 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K030143-022-864-C02C-8409
TTGGACAGTTTGTTTGTTGCCGAAAGTGCCAGGAGGAACACAAAGACATTTTTTGCAACA
CTTGAGGCAAAAGAACATGCATGGCTTCTTGTGTGATGTTGCTGAACAACGGAAGCTACA
CTTTGAGTTGCACTCTACAATTGAAGTTTCCATCAAAAAAGCATATCACAACAAACAAAC
AAAAAACAAATCATTGAGATCTTTATGTTCATGCTTATAATTCACAGCATACAGTATACT
AGAAAACTGACATTTCACAAAATAAACACCATTATTGTTACTTTAAAGTTTATTTATCAA
CATTATCAATAATTAGTGATTAATAAATTAATATGATCATATGAAACAACAAAAGTTTTG
AGTCCGTACGTTGGGGTTTGAGTTTGCCACCACCACGAGCAGCCTGTCATATATATATAA
AACTTCAATATCAGAAAACTAATAGCCATTAAATTTTTAAGGAATGTATAGCCTTTGGTC
ATTTTTACCTCCACGAGCAGCCTGTAATATATAAAACTTCAATATCAGACTTTAATAAAC
TGAATCTGTTTTGAGTGGTTTACCTGAACGAGGTTGGAGACTGACAATAAAAAGAGAAGA
GTCAAGAAGAAAAGAGCATTTCTTCTGATACAATTC
   
Sequence Name 41-K030143-022-864-C02C-DB19
GenBank Accession LN503961 [GenBank]
Sequencing primer gene specific primer, DB19: 5'-AAAAATGGCGAATTGTATCAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K030143-022-864-C02C-DB19
TCTTTTATTGTCAGTCTCCAACCTCGCTTCAGGTAAACCACTCAAAACAGATTCAGTTTA
TTAAAGTCTGATATTGAAGTTTTATATATTACAGGCTGCTCGTGGAGGTAAAAATGACCA
AAGGCTATACATTCCTTAAAAATTTAATGGCTATTAGTTTTCTGATATTGAAGTTTTATA
TATATATGACAGGCTGCTCGTGGTGGTGGCAAACTCAAACCCCAACGTACGGACTCAAAA
CTTTTGTTGTTTCATATGATCATATTAATTTATTAATCACTAATTATTGATAATGTTGAT
AAATAAACTTTAAAGTAACAATAATGGTGTTTATTTTGTGAAATGTCAGTTTTCTAGTAT
ACTGTATGCTGTGAATTATAAGCATGAACATAAAGATCTCAATGATTTGTTTTTTGTTTG
TTTGTTGTGATATGCTTTTTTGATGGAAACTTCAATTGTAGAGTGCAACTCAAAGTGTAG
CTTCCGTTGTTCAGCAACATCACACAAGAAGCCATGCATGTTCTTTTGCCTCAAGTGTTG
CAAAAAATGTCTTTGTGTTCCTCCTGGCACTTTCGGCAACAAACAAACTTGTCCAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11