Sequences from confirmation amplicon


Confirmation sequences for line 864E08 and hit At1g10640 are shown.
Line ID 864E08
Locus At1g10640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3516293
Primers used for PCR 23qs - 8474, show primer details
   
Sequence Name 25-K115635-0022-864-E08C-23qs
GenBank Accession LN497593 [GenBank]
Sequencing primer gene specific primer, 23qs: 5'-CAGGTGAGAATTGATCTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115635-0022-864-E08C-23qs
GCTTTGTTGTGAATTATGCAGCTTGATGGTACGATTATAGCTCCAACGGATTCAAAGACA
TGGGGAAAAGGGTTAATGTGGTGGATTGATTTTACAAAGCTGAAAGGAATTAAAGTACAA
GGGAAAGGTGTTATTGATGGAAGAGGCTCTGGTTGGTGGCAACAAGATTCTCCTTTCATT
GATAGTGATACCAAACTCATCGTCCCTTTGAACAATTCTGCTAACCAAAACCCTCCTATG
CCGGTAAGTTCATCGTCTTCTTCTTCACTGTCTCATTCACAACACAATATTCATCTAAGA
CCAAACTCTGTTTTGTTTTTACATATGACAGATCAGAAGTGAGCTTGATGAGAGAATGCC
AAGCATTAAACCAACGGTGAGAAATAGATATTGGCACTGTGAGATTGTAAGCATTAGTTT
GTTTACTGATTTTTGTTTCTGTTAGGCATTGAGATTCTCTGGGAGTTTTGGTGTGGAAGT
GACGGGTATAACGATACAAAACAGTCCTCAGTGTCACCTCAAATTCGACGACTGCGTATG
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 11-K115634-0022-864-E08C-8409
GenBank Accession LN491259 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115634-0022-864-E08C-8409
TTTGTATCGTTATACCCGTCACTTCCACACCAAAACTCCCAGAGAATCTCAATGCCTAAC
AGAAACAAAAATCAGTAAACAAACTAATGCTTACAATCTCACAGTGCCAATATCTATTTC
TCACCGTTGGTTTAATGCTTGGCATTCTCTCATCAAGCTCACTTCTGATCTGTCATATGT
AAAAACAAAACAGAGTTTGGTCTTAGATGAATATTGTGTTGTGAATGAGACAGTGAAGAA
GAAGACGATGAACTTACCGGCATAGGAGGGTTTTGGTTAGCAGAATTGTTCAAAGGGACG
ATGAGTTTGGTATCACTATCAATGAAAGGAGAATCTTGTTGCCACCAACCAGAGCCTCTT
CCATCAATAACACCTTTCCCTTGTACTTTAATTCCTTTCAGCTTTGTAAAATCAATCCAC
CACATTAACCCTTTTCCCCATGTCTTTGAATCCGTTGGAGCTATAATCGTACCATCAAGC
TGCATAATTCACAACAAAGCTTAGAATATTTTCACCAAAGTGATACACTTCTATATAAAA
GAG

Last Updated on 06.12.2018 16:11