Sequences from confirmation amplicon


Confirmation sequences for line 864F04 and hit At4g10440 are shown.
Line ID 864F04
Locus At4g10440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6460311
Primers used for PCR HM97 - 8474, show primer details
   
Sequence Name 65-K101328-0022-864-F04C-8409
GenBank Accession LN511355 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K101328-0022-864-F04C-8409
TTTACATTGAATATATCCTGAAGATTTCCGAATTAGAATAATTTGTTTATTGCTTTCTTT
TTCTCAGGGACTATCTTCTTGTAATGTGCTATTCTCTCTTTCCAAACCTCGTTGTCTTCT
CTAAATTTCTCCGCGTTCATTTCTGGTATAGTTCCTCTGATGATTCTTGGAGGTACCGCG
AATGCTCGGTCTGGCCAATCCTCGAGTGCACCGCCTGCTGAATCATCTGGATTGTTTGTT
TCTGGTAATGGTGTTATACAAGTTTCCAAGTCTTTGTACCTAAAACAGAGAAAACCAAGT
TTTGACATTGTTCTTTCACAAAGTTAAGACTACATTTTCGAGATGTAAATTAGTATTACC
AAGCGGAATCCGCGTTATCTGAGCTGCATATCGGAGGTG
   
Sequence Name 65-K101334-0022-864-F04C-HM97
GenBank Accession LN511356 [GenBank]
Sequencing primer gene specific primer, HM97: 5'-AACAATAAGTCACCTCCGATATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K101334-0022-864-F04C-HM97
CGCTTGGTATACAATTTCATCTCGAAATGTAGTCTTAACTTTGTGAAAGACCTTGTCAAA
ACTTGGTTTTCTCTGTTTTAGGTACAAAGACTTGGAAACTTGTATAACACCATTACCAGA
AACAAACAATCCAGATGATTCAGCAGGCGGTGCACTCGAGGATTGGCCAGACCGAGCATT
CGCGGTACCTCCAAGAATCATCAGAGGAACTATACCAGAAATGAACGCGGAGAAATTTAG
AGAAGACAACGAGGTTTGGAAAGAGAGAATAGCACATTACAAGAAGATAGTCCCTGAGAA
AAAGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT

Last Updated on 06.12.2018 16:11