Sequences from confirmation amplicon


Confirmation sequences for line 864F07 and hit At5g21090 are shown.
Line ID 864F07
Locus At5g21090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7165953
Primers used for PCR 08tj - 8474, show primer details
   
Sequence Name 24-K116871w-0022-864-F07C-08tj
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 08tj: 5'-GAGACAATGTATGAAGAGGCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K116871w-0022-864-F07C-08tj
ACACCCCATAAACAATCTTGTTCTCTTACTCAACATTTGATAAATCATTTTGCAATTTTT
AAAGAAGTCTTGAGTATATTGTTCAATGTGTCTGTATCAGAGTTTGAGAGAAACTTACAA
AAAGACCAGAGACTTCAATTTTCCCAAAGAAGTGGGAACTATCCCTGTAAGATTGTTGTT
GTACAGATCCAAGCTGATGAGATTCTTCAGATTTCCAAGTTCGGAAGGTATAGTTCCTTG
GATGTTGTTTTTGTAGAGCTCTCTGCATCAATCATAGGACAAGTTAAGAGTTAGTCTTAC
ATTGTCTATAACATAACCAATCTCAAAGTAAACAGTTTATTATAGGATAATATGGTATAC
AATATGGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCC
   
Sequence Name 04-K116870w-0022-864-F07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116870w-0022-864-F07C-8409
ATTTACATTGAATATATTCCATATTGTATACCATATTATCCTATAATAAACTGTTTACTT
TGAGATTGGTTATGTTATAGACAATGTAAGACTAACTCTTAACTTGTCCTATGATTGATG
CAGAGAGCTCTACAAAAACAACATCCAAGGAACTATACCTTCCGAACTTGGAAATCTGAA
GAATCTCATCAGCTTGGATCTGTACAACAACAATCTTACAGGGATAGTTCCCACTTCTTT
GGGAAAATTGAAGTCTCTGGTCTTTTTGTAAGTTTCTCTCAAACTCTGATACAGACACAT
TGAACAATATACTCAAGACTTCTTTAAAAATTGCAAAATGATTTATCAAATGTTGAGTAA
GAGAACAAGATTGTTTATGTGGGTTGTTCTCCAATACCTGATTCTTTTTTTGTGATGCCT
CTTCATACATTGTCTCAGNNGGAGGGGTG

Last Updated on 06.12.2018 16:11