Sequences from confirmation amplicon


Confirmation sequences for line 864G09 and hit At3g09480 are shown.
Line ID 864G09
Locus At3g09480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2915168
Primers used for PCR HM26 - 8474, show primer details
   
Sequence Name 03-K032598-022-864-G09C-8409
GenBank Accession LN487370 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K032598-022-864-G09C-8409
ACAATTGAAGACAAGAGCACAGAGACGTACAAGATCTACCTATTCAAGGTGTTGAAACAA
GTTCATCCTGATATCGGGATCTCTGGAAAAGCCATGGGAATCATGAACAGTTTCATCAAC
GACACCTTCGAGAAAATTGCTCTGGAATCGTCGCGATTGGCTAGGTATAACAAGAAACCG
ACGATCACGTCCCGGGAAATCCAAACCGCCGTAAGGCTCGTGTTGCCTGGTGAATTAGCG
AAGCACGCTGTTTCTGAGGGTACCAAAGCCGTCACCAAATTCACCAGTTCTTAGATTAGG
TTCTTTTTTTCTCTTCGATTTTATTTTCGGTTAATATGTTCACCAGTTGATTTCAAAGAT
ATAATTTTCGGCAGTCTCTTCGATATTTTCTATGATTGATTTTACGTTATGATGCTATTG
AAATTAGGGTTTACAAATCTTTGGGTATAATAAATACAAAATTGTAAAGGATCTCGTTTC
TTCCTCAGTTTCCCCGGGAAATAAACGGAAATTATAAATCTTGAGATTCTTTAATTGGCC
CAGCCCAACAAACACCAATCTCAATTTAGTCTGAACCGATATAAATTGAAATTAGAAATA
CCGTACCAAAATAAACCGAACCGCAATCATCACCGGTCTTGGAA
   
Sequence Name 03-K032598-022-864-G09C-HM26
GenBank Accession LN487371 [GenBank]
Sequencing primer gene specific primer, HM26: 5'-TACCGTTTCAGAGATTTGGAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K032598-022-864-G09C-HM26
GTTATTTTGGTCGGTATTTCTATTTCATTTATATCGGTTCAGACTAAATTGAGATTGGTG
TTTGTTGGGCTGGGCCAATTAAAGAATCTCAAGATTTATAATTTCCGTTTATTTCCCGGG
GAAACTGAGGAAGAAACGAGATCCTTTACAATTTTGTATTTATTATACCCAAAGATTTGT
AAACCCTAATTTCAATAGCATCATAACGTAAAATCAATCATAGAAAATATCGAAGAGACT
GCCGAAAATTATATCTTTGAAATCAACTGGTGAACATATTAACCGAAAATAAAATCGAAG
AGAAAAAAAGAACCTAATCTAAGAACTGGTGAATTTGGTGACGGCTTTGGTACCCTCAGA
AACAGCGTGCTTCGCTAATTCACCAGGCAACACGAGCCTTACGGCGGTTTGGATTTCCCG
GGACGTGATCGTCGGTTTCTTGTTATACCTAGCCAATCGCGACGATTCCAGAGCAATTTT
CTCGAAGGTGTCGTTGATGAAACTGTTCATGATTCCCATGGCTTTTCCAGAGATCCCGAT
ATCAGGATGAACTTGTTTCAACACCTTGAATAGGTAGATCTTGTACGTCTCTGTGCTCTT
AGTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAATCTTTATNTCGA
GTGTCTACT
Primers used for PCR FQ33 - 8474, show primer details
   
Sequence Name 66-K118414-0022-864-G09C-FQ33
GenBank Accession LN511705 [GenBank]
Sequencing primer gene specific primer, FQ33: 5'-TGCTTCTGAGAAATCCCAATAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K118414-0022-864-G09C-FQ33
GACAGAGCAGATTAAGAAGAGCAGAGCAATAAGTTGGAAATCGACGAAATTTTTTAAAAG
GATGCCGTCTTGTAAAAGGCCAAGGCCCATTAGATGAGCCCACGATTAGTTCGGTTCCTC
TCGTGAATTCAACCGTAGATTATCTTGTATCAACGCACGTGATAACGCCACGTAGACGAT
CCGTGAGATTGATCAAAAACCAATCATGTTCGTATCAGCACCTCTATATATATCTCGGTA
TCACTCGGAGAGCGATCATTAATCGTCAATTTACAACTAGTCGCGAAATCCGAATTTTAT
CCAAAGCAATGGCACCGAAGGCAGAGAAGAAACCGTCGGAGAAAGCACCAAAGGCAGATA
AGAAGATCACGAAAGAAGGAGGAAGCGAGAGGAAGAAGAAGACTAAGAAGAAAAAAGGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAAAGTTTATT
   
Sequence Name 18-K118413-0022-864-G09C-8409
GenBank Accession LN494512 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118413-0022-864-G09C-8409
TCATTGAATTATCCTGCCTTTTTTCTTCTTAGTCTTCTTCTTCCTCTCGCTTCCTCCTTC
TTTCGTGATCTTCTTATCTGCCTTTGGTGCTTTCTCCGACGGTTTCTTCTCTGCCTTCGG
TGCCATTGCTTTGGATAAAATTCGGATTTCGCGACTAGTTGTAAATTGACGATTAATGAT
CGCTCTCCGAGTGATACCGAGATATATATAGAGGTGCTGATACGAACATGATTGGTTTTT
GATCAATCTCACGGATCGTCTACGTGGCGTTATCACGTGCGTTGATACAAGATAATCTAC
GGTTGAATTCACGAGAGGAACCGAACTAATCGTGGGCTCATCTAATGGGCCTTGGCCTTT
TACAAGACGGCATCCTTTTTAAAAAATTTCGTCGATTTCCAACTTATTTGCTCTGCTCTT
CTTTAATCTGCTCTGTTCTTCTCTTTCCATTTCAGCGTTGACTTAGTTATTGGGATTTCT
CAGAAGC

Last Updated on 06.12.2018 16:11