Sequences from confirmation amplicon


Confirmation sequences for line 864H04 and hit At4g35600 are shown.
Line ID 864H04
Locus At4g35600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16897639
Primers used for PCR EP36 - 8474, show primer details
   
Sequence Name 78-K103232-0022-864-H04C-EP36
GenBank Accession LN513674 [GenBank]
Sequencing primer gene specific primer, EP36: 5'-GCTATTGAATTCGGTGTAGCTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K103232-0022-864-H04C-EP36
GGAACGTTGGTACATGTTATTAGCTCATAACATTGTAGTCAAAGTATAATTCATTATAAT
GTAATATAATTAATTTAAAATTAGTAAAAGGTTATTAGAAAGTCAACATTATTTCTCTGT
TGCCTCGTATTGGATGCAAATCTGGATTCTTTCATTGAGTTTATTGTATGTCTTAATTAG
CAGAATAGATTAATCCTTCAAGTTATATAGGCTAAATTTGAGTTTTATGGCTTTGTTTGT
TTTTTGGACAGTCAGAAGTTAACTTCTTGGGAATGCTTTCGCACCGAAATCTGGTGAAGT
TATTGGGATACTGTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACNTTCACGTCCAAATGG
   
Sequence Name 30-K103231-0022-864-H04C-8409
GenBank Accession LN499676 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103231-0022-864-H04C-8409
GCCATTTACCAGTATCCCAATAACTTCACCAGATTTCGGTGCGAAAGCATTCCCAAGAAG
TTAACTTCTGACTGTCCAAAAAACAAACAAAGCCATAAAACTCAAATTTAGCCTATATAA
CTTGAAGGATTAATCTATTCTGCTAATTAAGACATACAATAAACTCAATGAAAGAATCCA
GATTTGCATCCAATACGAGGCAACAGAGAAATAATGTTGACTTTCTAATAACCTTTTACT
AATTTTAAATTAATTATATTACATTATAATGAATTATACTTTGACTACAATGTTATGAGC
TAATAACATGTACCAACGTGTCCCCAACTCAAAAGTCCGGTAACAACGGAAAGTTCCTAG
CTACACCGAATTCAATAG

Last Updated on 06.12.2018 16:11