Sequences from confirmation amplicon


Confirmation sequences for line 865B01 and hit At1g24822 are shown.
Line ID 865B01
Locus At1g24822 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8777651
Primers used for PCR JF18 - 8474, show primer details
   
Sequence Name 46-K102502-0022-865-B01C-8409
GenBank Accession LN505826 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K102502-0022-865-B01C-8409
ATTTACATTGCCTCGATTTCCAAATTCACAGATCTACTCTAATTTTAAATACATTCCTCC
AATTCCCAAACTCACCAATGTTTCTTGATACTTTCAATTTCAATTTCTTTATTACCCCAA
ATTCGCTACCCCTATATCTTGCTTCTGAGAATATAATCGTTTTTTATTACCTTCATTTCC
TTCAGGTAGGTCCATATGCTGAAGATTTATCGCAGGAGCGACCACTACGGGAGCCGCCAT
TGGAGATTCTCTGTGAGAGAGAGAGAGAGAAATAGTTTGAGAATCAATCTTCTGTATTTT
ATTGTGTAGATCAAGTTATTTAGTATATGCTAGTACTTATGGTTAAAATAGACTATTATT
AAAATTCAAAAGTAATAAAGCCAACGAGTACACTAAACTCAGATTATATTTTCCAAAACT
ATTGCTAAAGAATATCAATACGTACACTAGATATAGAAATCGGAATTAGGTACGTTGTGA
TCCATTTCAGCTACTGTTAGGGGTAAGATTTGGGCTTGCTTGGCCCAAGGCCCAAATCCA
AGTCAAAGGCCTAAGCAGAATGTTAAGTCAAGAGGGTAGGCTCGTCAAAATCCTGGAGGA
GTGTCCTACACGCAG
   
Sequence Name 46-K102503-0022-865-B01C-JF18
GenBank Accession LN505827 [GenBank]
Sequencing primer gene specific primer, JF18: 5'-ATTTATAGCCTTCTGCGTGTAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K102503-0022-865-B01C-JF18
CTCTTGACTTAACATTCTGCTTAGGCCTTTGACTTGGATTTGGGCCTTGGGCCAAGCAAG
CCCAAATCTTACCCCTAACAGTAGCTGAAATGGATCACAACGTACCTAATTCCGATTTCT
ATATCTAGTGTACGTATTGATATTCTTTAGCAATAGTTTTGGAAAATATAATCTGAGTTT
AGTGTACTCGTTGGCTTTATTACTTTTGAATTTTAATAATAGTCTATTTTAACCATAAGT
ACTAGCATATACTAAATAACTTGATCTACACAATAAAATACAGAAGATTGATTCTCAAAC
TATTTCTCTCTCTCTCTCTCACAGAGAATCTCCAATGGCGGCTCCCGTAGTGGTCGCTCC
TGCGATAAATCTTCAGCATATGGACCTACCTGAAGGAAATGAAGGTAATAAAAAACGATT
ATATTCTCAGAAGCAAGATATAGGGGTAGCGAATTTGGGGTAATAAAGAAATTGAAATTG
AAAGTATCAAGAAACATTGGTGAGTTTGGGAATTGGAGGAATGTATTTAAAATTAGAGTA
GATCTGTGAATTTGGGAAATCGAGGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11