Sequences from confirmation amplicon


Confirmation sequences for line 865D07 and hit At3g11440 are shown.
Line ID 865D07
Locus At3g11440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3604322
Primers used for PCR 59hw - 8474, show primer details
   
Sequence Name 45-K112810-0022-865-D07C-59hw
GenBank Accession LN505588 [GenBank]
Sequencing primer gene specific primer, 59hw: 5'-CGATCTAAATTTTGCTGCTAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K112810-0022-865-D07C-59hw
GCTGCACATGCTAGGAACTGGCGCAAGTTCTTCCCGGTATGAGAGCTACATGCCACCAAT
ATTGCCTTCCCCAAAGCAAATCTGGGAATCTGGATCTCGGTTTCCCATGTGCAGCAGTAA
CATAAAGCATGAATTCCAATCGCCGGAACACTTTCAGAATACGGCTGTACAGAAGAATCC
CAGATCTTGCAGTATCTCGCCTTGTGATGTTGATCATCATCCTTATGAAAACCAACATTC
GTCTCATATGATGATGGTTCCTGATAGCCATACAGTTACGTATGGCATGCATCCTACTTC
TAAGCCCTTGTTTGGGGCAGTGAAGCTGGAGCTCCCTTCATTCCAATATTCAGAAACTAG
TGCATTTGATCAGTGGAAGACGACTCCGTCACCTCCACACTCAGATCTCCTTGACTCTGT
TGATGCCTATATTCAATCTCCACCACCATCGCAGGTAGAGGAGTCAGATTGTTTCTCTTC
ATGCGACACCGGCCTACTTTCTAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTA
   
Sequence Name 20-K112809-0022-865-D07C-8409
GenBank Accession LN495282 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K112809-0022-865-D07C-8409
CATTTCATTGATTAGAAGTAGGCCGGTGTCGCATGAAGAGAACAATCTGACTCCTCTACC
TGCGATGGTGGTGGAGATTGAATATAGGCATCAACAGAGTCAAGGAGATCTGAGTGTGGA
GGTGACGGAGTCGTCTTCCACTGATCAAATGCACTAGTTTCTGAATATTGGAATGAAGGG
AGCTCCAGCTTCACTGCCCCAAACAAGGGCTTAGAAGTAGGATGCATGCCATACGTAACT
GTATGGCTATCAGGAACCATCATCATATGAGACGAATGTTGGTTTTCATAAGGATGATGA
TCAACATCACAAGGCGAGATACTGCAAGATCTGGGATTCTTCTGTACAGCCGTATTCTGA
AAGTGTTCCGGCGATTGGAATTCATGCTTTATGTTACTGCTGCACATGGGAAACCGAGAT
CCAGATTCCCAGATTTGCTTTGGGGAAGGCAATATTGGTGGCATGTAGCTCTCATACCGG
GAAGAACTTGCGCCAGTTCCTAGCATGTTGCATGCAACCAAATCTGATAGGTCAGAAGCG
GCAGGTAACAAGCTAGCAGCAAATTTTAGATC

Last Updated on 06.12.2018 16:11