Sequences from confirmation amplicon


Confirmation sequences for line 865E01 and hit At2g19390 are shown.
Line ID 865E01
Locus At2g19390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8395572
Primers used for PCR 26dr - 8474, show primer details
   
Sequence Name 60-K110361-0022-865-E01C-26dr
GenBank Accession LN510287 [GenBank]
Sequencing primer gene specific primer, 26dr: 5'-GTATTGCTCTTGGAATACTAGGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K110361-0022-865-E01C-26dr
GAGTTATTCATCTCCTATTCAGAAGAGATCAAAAGATTTAAGGCTGGTCTGCGCGAAAAC
AATGTGAAGGCCAGGTAGTTTTTAACTACCACAAGTTAGTTAGTTGTGTTTGAGGTGCTC
TTTTGACTCATTTACTTTTTGTGGTCGCAGGGAGCGTGTAAAAATTTTCAACGAAGCTTC
TTCTGTATTTAACAAGTTTTTCCCAAGTGTACCTACAAAGAAAAGGTCTCGACCTGAAGG
GTTTTCTGGTGACCGCTCTGGTGATCGCTTGGTATCAGGACCAGGCCTAGGTAAAATGGG
AATTCAGGGTCAGACCCTGGCTGGTGGTTTTGAGCTTGACCAGCAAAAGTTGGATGAACG
ACCTAAAAGTGGTGTTCCAAATAAGCGAACACGTACTTCCATGGTAATTCTGATTTTTTT
TACATATGGAGTTGTTATCTTGGTTCGTACTTGAACAAATGTCTTATATTCTATAATGCA
GTCTTCTGTTTGCATGTCATTGCATGCTTCTGATCACTGGAAGGTGCATCTTTTGAATTG
GTCAAAAAATTGGATCGTGGTTACTAGTTTTAGATATGATATGCTCCAGTGTGTATTCCA
TTGTAAATGGCTTCATGTC
   
Sequence Name 44-K110360-0022-865-E01C-8409
GenBank Accession LN505217 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K110360-0022-865-E01C-8409
GCCATTTACAATTGAATACCACTGGAGCATATCATATCTAAAACTAGTAACCACGATCCA
ATTTTTTGACCAATTCAAAAGATGCACCTTCCAGTGATCAGAAGCATGCAATGACATGCA
AACAGAAGACTGCATTATAGAATATAAGACATTTGTTCAAGTACGAACCAAGATAACAAC
TCCATATGTAAAAAAAATCAGAATTACCATGGAAGTACGTGTTCGCTTATTTGGAACACC
ACTTTTAGGTCGTTCATCCAACTTTTGCTGGTCAAGCTCAAAACCACCAGCCAGGGTCTG
ACCCTGAATTCCCATTTTACCTAGGCCTGGTCCTGATACCAAGCGATCACCAGAGCGGTC
ACCAGAAAACCCTTCAGGTCGAGACCTTTTCTTTGTAGGTACACTTGGGAAAAACTTGTT
AAATACAGAAGAAGCTTCGTTGAAAATTTTTACACGCTCCCTGCGACCACAAAAAGTAAA
TGAGTCAAAAGAGCACCTCAAACACAACTAACTAACTTGTGGTAGTTAAAAACTACCTGG
CCTTCACATTGTTTTCGCGCAGACCAGCCTTAAATCTTTTGATCTCTTCTGGAATAGGAG
ATGGAATAAACTTCCCTTTTAAAGATCCACT

Last Updated on 06.12.2018 16:11