Sequences from confirmation amplicon


Confirmation sequences for line 866B08 and hit At3g46460 are shown.
Line ID 866B08
Locus At3g46460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17096136
Primers used for PCR KQ70 - 8474, show primer details
   
Sequence Name 03-K101499-0022-866-B08C-8409
GenBank Accession LN487420 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101499-0022-866-B08C-8409
GCCATTTACAATTGCTGATCCATCATACTCAAGAAATGTTTTGATCAAAGAAACACTTTC
ACACTCCAACACCATCTTCTATGGAGTTCAAAAGCTCATTTATCTTATCGAAAAAAATTC
CCTTTGAAAAACTGTCCAAACACTACTTGTAATAAACCCGTCTTGCATCTTTTAATCGCG
TTCCTCAACGTGAAATGAAGTGTTTGGTCGAAAAAAATGTTGTTCTTGAAAAAAACTGAC
ATTAAAAGTTATACTATTCTTCATAGAAGCTTTGTTTTTGTCTGTCGAAGCTTTGTTTTT
CAGTGTGTTTACTTCCTTGATTTGGACGCTAAAACTCCACGCCGTTCCTACGTTCTCCTC
AGAAACTTCGTACAGTTTTCGGCTTCTAGTAAAAATATTTAAACAAACAGCATGATGTTT
GTTTCTTGGGTCCACGTAATTA
   
Sequence Name 03-K101503-0022-866-B08C-KQ70
GenBank Accession LN487421 [GenBank]
Sequencing primer gene specific primer, KQ70: 5'-TTTAAATACGTGGACCCAAGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101503-0022-866-B08C-KQ70
ATATTTTTCTANAAGCCGAAACTGTACGAAGTTTCTGAGGAGAACGTAGGAACGGCGTGG
AGTTTTAGCGTCCAAATCAAGGAAGTAAACACACTGAAAAACAAAGCTTCGACAGACAAA
AACAAAGCTTCTATGAAGAATAGTATAACTTTTAATGTCAGTTTTTTTCAAGAACAACAT
TTTTTTCGACCAAACACTTCATTTCACGTTGAGGAACGCGATTAAAAGATGCAAGACGGG
TTTATTACAAGTAGTGTTTGGACAGTTTTTCAAAGGGAATTTTTTTCGATAAGATAAATG
AGCTTTTGAACTCCATAGAAGATGGTGTTGGAGTGTGAAAGTGTTTCTTTGATCAAAACA
TTTCTTGAGTATGATGGATCAGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11