Sequences from confirmation amplicon


Confirmation sequences for line 867E08 and hit At3g22720 are shown.
Line ID 867E08
Locus At3g22720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8029238
Primers used for PCR 44we - 8474, show primer details
   
Sequence Name 30-K118658-0022-867-E08C-44we
GenBank Accession LN499926 [GenBank]
Sequencing primer gene specific primer, 44we: 5'-CGACATTGTCTTCAAAAACAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118658-0022-867-E08C-44we
TTCTTTCGTTTCTTTTACGAATCTACCTTACTGGATTTGAACAAACTCGGAACATAATTG
AAAATCAATGAGTACCATCTTGTAGTATGGATAAGTTGAAGCCTTTCTATTTTTATAGAT
TTTACGAAAGGGATTTCTATGTAATGTCCATCGTCCTCTCCGATAATGTATACCACTTTC
TTGCTGGTCTCACCATCTTTAACATTACAATACAAGACGACTTTCTTCTCCTCGTCGAAA
TATAGACTTGAGAAGATTCGAACACAATTACATTGACTGTCTAAGTGCACTCTTTTCCAC
AACAATGTAGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
ACGGTTATT
   
Sequence Name 15-K118657-0022-867-E08C-8409
GenBank Accession LN493192 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118657-0022-867-E08C-8409
TGTTGTGGAAAGAGTGCACTTAGACAGTCAATGTAATTGTGTTCGAATCTTCTCAAGTCT
ATATTTCGACGAGGAGAAGAAAGTCGTCTTGTATTGTAATGTTAAAGATGGTGAGACCAG
CAAGAAAGTGGTATACATTATCGGAGAGGACGATGGACATTACATAGAAATCCCTTTCGT
AAAATCTATAAAAATAGAAAGGCTTCAACTTATCCATACTACAAGATGGTACTCATTGAT
TTTCAATTATGTTCCGAGTTTGTTTCAAATCCAGTAAGGTAGATTCGTAAAAGAAACGAA
AGAAACTAGTCCTTTTTTTTCTTTCTTAGTTTTTGTTTTTGAAGACAATGTCG

Last Updated on 06.12.2018 16:11