Sequences from confirmation amplicon


Confirmation sequences for line 867H11 and hit At3g12977 are shown.
Line ID 867H11
Locus At3g12977 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4144906
Primers used for PCR 07ux - 8474, show primer details
   
Sequence Name 94-K117671-0022-867-H11C-07ux
GenBank Accession LN515316 [GenBank]
Sequencing primer gene specific primer, 07ux: 5'-AAATATGTTGGAGAAAATACCCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K117671-0022-867-H11C-07ux
GTTAGTATGTACTATGTAATATACAAAGAAAAACATTCAACGCCCAACTTCTTATCAATA
GTTAAGTTTGATAGTATATAAAAAGGTCATTTTCAGAAACGGCGAGAGTGGGAGGGAAAG
AATGGTACTTTTACAGCCAAAAAGACCGTAAATACGCAACAGGCTACAGAACAAACCGGG
CTACGGCCACCGGTTATTGGAAAGCCACCGGGAAAGATAGAGCAATCCAAAGAAACGGTG
GTCTTGTGGGTATGAGAAAGACACTTGTGTTTTACCGAGGTCGATCCCCTAAAGGTCGTA
AAACTGATTGGGTCGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTAT
   
Sequence Name 46-K117670-0022-867-H11C-8409
GenBank Accession LN505993 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117670-0022-867-H11C-8409
TTGAATAATCCTGTCCGACCCAATCAGTTTTACGACCTTTAGGGGATCGACCTCGGTAAA
ACACAAGTGTCTTTCTCATACCCACAAGACCACCGTTTCTTTGGATTGCTCTATCTTTCC
CGGTGGCTTTCCAATAACCGGTGGCCGTAGCCCGGTTTGTTCTGTAGCCTGTTGCGTATT
TACGGTCTTTTTGGCTGTAAAAGTACCATTCTTTCCCTCCCACTCTCGCCGTTTCTGAAA
ATGACCTTTTTATATACTATCAAACTTAACTATTGATAAGAAGTTGGGCGTTGAATGTTT
TTCTTTGTATATTACATAGTACATACTAACATACATTAATGGATTTTAGTTTTCTATTAA
GGGAGGGGGTATTTTCTCCCACATATTT

Last Updated on 06.12.2018 16:11