Sequences from confirmation amplicon


Confirmation sequences for line 868A02 and hit At2g44390 are shown.
Line ID 868A02
Locus At2g44390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18325844
Primers used for PCR 88qa - 8474, show primer details
   
Sequence Name 50-K115305-0022-868-A02C-88qa
GenBank Accession LN507271 [GenBank]
Sequencing primer gene specific primer, 88qa: 5'-TTTGTGAAGAAGATATGTCGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K115305-0022-868-A02C-88qa
GGGACGCATGTGCATTCTTGCGCGGTTTATGAAGACGATGAGCCAAATAATAGAGGAGGA
GAAGAAGGAGAAGCGATGAGCTCGGCGGTTTCGAGGATGAAGTCGTTGATGAAGGCTGAG
GATGAGTTGGCGGCGGTACAGCTCGAGGCACGTATGAAGCGAGACGCTAATAATGCTATA
CTCGGTTTAAATGGCTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGC
   
Sequence Name 15-K115304-0022-868-A02C-8409
GenBank Accession LN493103 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115304-0022-868-A02C-8409
GCATTTCATTGAGCCATTTAAACCGAGTATAGCATTATTAGCGTCTCGCTTCATACGTGC
CTCGAGCTGTACCGCCGCCAACTCATCCTCAGCCTTCATCAACGACTTCATCCTCGAAAC
CGCCGAGCTCATCGCTTCTCCTTCTTCTCCTCCTCTATTATTTGGCTCATCGTCTTCATA
AACCGCGCAAGAATGCACATGCGTCCCATAATCACATTCTTTGCAATAATAAACCCATAG
ATTCTCCGACATATCTTCTTCACAAAA

Last Updated on 06.12.2018 16:11