Sequences from confirmation amplicon


Confirmation sequences for line 868B10 and hit At1g75900 are shown.
Line ID 868B10
Locus At1g75900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28500176
Primers used for PCR JC16 - 8474, show primer details
   
Sequence Name 13-K101743-0022-868-B10C-8409
GenBank Accession LN491976 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101743-0022-868-B10C-8409
ATTTACATTGAATTATCCTGCCATACATACTATACTATACATACACTACGTGCAATACAT
ACTCATTGCTACTATGAAGTCTTTTCTTACATACTATACTCTACGTGCAAGACCTGAATA
CGACGTCGATTCATACACTACTCTTATGTCTGACTCTGCCTCAGAATTTGTGACTGTAAG
CTTCTCTCATATTTTACTAAAAAGCCTAATACGTTTGATATATATACATTATACTAAATA
ACATTTTTAATAGACGGCTATATTTCTAGGTAATTAGTATAATGTTTAGTGTGAAATGAA
ATTGGCAGAAACTATATGGATATGGAGTGAGAAGAGTAGCTGTGTTTGGTGCACCACCAA
TTGGGTGTGTACCATCACAGAGAACGTTAGGAGGAGGTATCTTGAGAGATTGTGCTGATA
ATTACNACGAAGC
   
Sequence Name 13-K101749-0022-868-B10C-JC16
GenBank Accession LN491977 [GenBank]
Sequencing primer gene specific primer, JC16: 5'-AAAGTTTTGCTGCTTCGTTGTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101749-0022-868-B10C-JC16
TCCTCCTAAGTTCTCTGTGATGGTACACACCCATTGGTGGTGCACCAAACACAGCTACTC
TTCTCACTCCATATCCATATAGTTTCTGCCAATTTCATTTCACACTAAACATTATACTAA
TTACCTAGAAATATAGCCGTCTATTAAAAATGTTATTTAGTATAATGTATATATATCAAA
CGTATTAGGCTTTTTAGTAAAATATGAGAGAAGCTTACAGTCACAAATTCTGAGGCAGAG
TCAGACATAAGAGTAGTGTATGAATCGACGTCGTATTCAGGTCTTGCACGTAGAGTATAG
TATGTAAGAAAAGACTTCATAGTAGCAATGAGTATGTATTGCACGTAGTGTATGTATAGT
ATAGTATGTATTGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11