Sequences from confirmation amplicon


Confirmation sequences for line 868C03 and hit At2g35840 are shown.
Line ID 868C03
Locus At2g35840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15055539
Primers used for PCR IF72 - 8474, show primer details
   
Sequence Name 59-K101502-0022-868-C03C-8409
GenBank Accession LN509950 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K101502-0022-868-C03C-8409
CATTTACAATTGAATATATGAGTTATAGATGATGCAGGTTTAGGCAATAAGGCTTAGCTT
ACCATCTTGCTCCCATTTATCAAGCTTTACTATCCATGTCCCAGGAGTAGTGTCTGTTGC
CAGGACTTGATCTGCCCAAACGCGAAACTTCTTCCCTTGTTTGTCTCCATGGTACTTGCG
CAGCTCATCGATGGTATCTCTCAGAGATTTTTCAGTACCAGATGGATGGACAAACACACC
ACCGGGGTGCTGAAAGAGGTTGACACAACAAGAGTCAGGTATTTAATCCAACTGCAATAC
ATTATCTAAATTCGGTTATATACTCACAACTGAAGCTTTGAGGCTTGCTGTGTATGCCTC
AGAGTTCTCAACCTCGCCCCGTCTCCATCTCTCGTAGAACAAAAAAAACTTCACAACCTC
GTGACCAGGGTTCACATTCTCCACCTTGATCTCTAAGAAATCAGAGACATCTCTNGGAGA
AAGGTTCGGACCAAGCTTGAAGTGACCAATGGCT
   
Sequence Name 59-K101506-0022-868-C03C-IF72
GenBank Accession LN509951 [GenBank]
Sequencing primer gene specific primer, IF72: 5'-GGATTATACAAGCCATTGGTCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K101506-0022-868-C03C-IF72
ACTTTTCAGAGAGTCTCTGATTTCTTAGAGATCAGGTGGAGAATGTGAACCCTGGTCACG
AGGTTGTGAAGTTTTTTTTGTTCTACGAGAGATGGAGACGGGGCGAGGTTGAGAACTCTG
AGGCATACACAGCAAGCCTCAAAGCTTCAGTTGTGAGTATATAACCGAATTTAGATAATG
TATTGCAGTTGGATTAAATACCTGACTCTTGTTGTGTCAACCTCTTTCAGCACCCCGGTG
GTGTGTTTGTCCATCCATCTGGTACTGAAAAATCTCTGAGAGATACCATCGATGAGCTGC
GCAAGTACCATGGAGACAAACAAGGGAAGAAGTTTCGCGTTTGGGCAGATCAAGTCCTGG
CAACAGACACTACTCCTGGGACATGGATAGTAAAGCTTGATAAATGGGAGCAAGATGGTA
AGCTAAGCCTTATTGCCTAAACCTGCATCATCTATAACTCATATTTTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG

Last Updated on 06.12.2018 16:11