Sequences from confirmation amplicon


Confirmation sequences for line 868C08 and hit At5g67190 are shown.
Line ID 868C08
Locus At5g67190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26809146
Primers used for PCR PC11 - 8474, show primer details
   
Sequence Name 95-K037023-022-868-C08C-8409
GenBank Accession LN515333 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K037023-022-868-C08C-8409
CATTTACAATTGACCATCATATTTCCCATTCTTCATCGGAGCTTCCCTGGTCGGGTAATT
TATTCAAGTCAACCCGATCCAATAACCGGCCGCCACTCCTCAATCTCTTGTCATCCTCAT
CTTCTTCTTCTTCTTCTCGTTCACCGTTTTGATAATTCCGATGAAAATTCTTATTATCCA
CATCACTATCTCGATTCTGACCAAAAACACGGTGGCGGTTATTTTGCACCGCCGTGCCCA
AAGCATCAACCTGAGCACCGACCTCCGTGGCTTTCTTCCTGATGGTCGCAGCCGACATAT
CCTCGTCGGAGAATTTCTCCCCAGGAAGAAGCTCAGGGAAGTTAAGCCTCGCCGTAGGTC
CTCTAAGATAGAAAACCGCCGTGTCGTAAGCTCGCGCCGCCGCCTCGGGAGTAGAGTAAG
AGCCAAGCCATAACCTAGAGCGCTTGTTAGGCTCACGAATCTCCGCCACCCACTTTCCCC
ACTTCCTCATCCTAATTCCTTTGTAAGGTCTGTCTCTCTTCCTCGTACCGGGGACAGCCA
CGTCAGCAACTCCGCCGCCTTCCATGGAGGATCTAAACCCTCGAAAGAGAAAGACTTTCG
AGATGGGTTTTGAGAGAGAGAGAGAGGCAGAGAATAAATATGTCAGAGCAGGAAAGAGAA
GGAGGGAGCTTGCAGGTGGTATTATGGTGATGAAAA
   
Sequence Name 96-K037023-022-868-C08C-PC11
GenBank Accession LN515372 [GenBank]
Sequencing primer gene specific primer, PC11: 5'-TCCTCTTTCCATCACCATAATACC-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K037023-022-868-C08C-PC11
TTTCTGCTCGACATATTTATTCTCTGCCTCTCTCTCTCTCTCAAAACCCATCTCGAAAGT
CTTTCTCTTTCGAGGGTTTAGATCCTCCATGGAAGGCGGCGGAGTTGCTGACGTGGCTGT
CCCCGGTACGAGGAAGAGAGACAGACCTTACAAAGGAATTAGGATGAGGAAGTGGGGAAA
GTGGGTGGCGGAGATTCGTGAGCCTAACAAGCGCTCTAGGTTATGGCTTGGCTCTTACTC
TACTCCCGAGGCGGCGGCGCGAGCTTACGACACGGCGGTTTTCTATCTTAGAGGACCTAC
GGCGAGGCTTAACTTCCCTGAGCTTCTTCCTGGGGAGAAATTCTCCGACGAGGATATGTC
GGCTGCGACCATCAGGAAGAAAGCCACGGAGGTCGGTGCTCAGGTTGATGCTTTGGGCAC
GGCGGTGCAAAATAACCGCCACCGTGTTTTTGGTCAGAATCGAGATAGTGATGTGGATAA
TAAGAATTTTCATCGGAATTATCAAAACGGTGAACGAGAAGAAGAAGAAGAAGATGAGGA
TGACAAGAGATTGAGGAGTGGCGGCCGGTTATTGGATCGGGTTGACTTGAATAAATTACC
CGACCCGGAAAGCTCCGATGAAGAATGGGAAATATGATGGTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAGAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTAC

Last Updated on 06.12.2018 16:11