Sequences from confirmation amplicon


Confirmation sequences for line 868E07 and hit At1g75590 are shown.
Line ID 868E07
Locus At1g75590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28383272
Primers used for PCR CT15 - 8474, show primer details
   
Sequence Name 74-K105358-0022-868-E07C-CT15
GenBank Accession LN513076 [GenBank]
Sequencing primer gene specific primer, CT15: 5'-TATAAACCTCGTCCCTCCCTAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K105358-0022-868-E07C-CT15
CAAGAAACCAATCACTAAACCGGAAAAAATGGCTGGAGGTCTCCGGAAATGCAGCAAGAT
CCGTCACATTGTGAGGCTAAGGCAAATGCTTCGACGGTGGCGCGATCAAGCGAGGATGTC
TTCTTCTTTCAGCCGTTGTGTACCGTCGGATGTACCGTCAGGACACGTGGCGGTCTACGT
AGGAAGCAGTTGTAGGAGATTTGTGGTGCGCGCGACGTATCTGAACCATCCTGTCCTAAG
GAACCTTCTTGTTCAAGCTGAGGAAGAGTTTGGTTTCGTTAACCAAGGTCCGTTGGTTAT
CCCTTGTGAAGAATCGGTTTTCGAGGAATCGATTCGGTTTATATCCCGGTCTGATTCGAC
CCGGTCAAGACGGTTTACTTGTCCCGATGATTTTCAGAAGAATTGCCACGTGGTGGGAAT
CAGAAGCAAGCTTGATCTATGGATCGAATCTCGACCGTTACTTCACGGCGTCACAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGNTTATT
   
Sequence Name 58-K105359-0022-868-E07C-8409
GenBank Accession LN509704 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K105359-0022-868-E07C-8409
TCATTGAATATATCCTGTGACGCCGTGAAGTAACGGTCGAGACTCGATCCATAGATCAAG
CTTGCTTCTGATTCCCACCACGTGGCAATTCTTCTGAAAATCATCGGGACAAGTAAACCG
TCTTGACCGGGTCGAATCAGACCGGGATATAAACCGAATCGATTCCTCGAAAACCGATTC
TTCACAAGGGATAACCAACGGACCTTGGTTAACGAAACCAAACTCTTCCTCAGCTTGAAC
AAGAAGGTTCCTTAGGACAGGATGGTTCAGATACGTCGCGCGCACCACAAATCTCCTACA
ACTGCTTCCTACGTAGACCGCCACGTGTCCTGACGGTACATCCGACGGTACACAACGGCT
GAAAGAAGAAGACATCCTCGCTTGATCGCGCCACCGTCGAAGCATTTGCCTTAGCCTCAC
AATGTGACGGATCTTGCTGCATTTCCCGAGACCTCCAGCCATTTTTTCCGGTTTAGTGAT
TTGGTTTTCTTTGTGAGAGAAAGAAAATGTCAGGGGGATTTTGAGAGTTAGGGAGGGACG
AGGTTTATA

Last Updated on 06.12.2018 16:11