Sequences from confirmation amplicon


Confirmation sequences for line 868G08 and hit At5g67280 are shown.
Line ID 868G08
Locus At5g67280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26843730
Primers used for PCR DR57 - 8474, show primer details
   
Sequence Name 18-K035539-022-868-G08C-DR57
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, DR57: 5'-CTCGGGATTGTGTTTTTCTACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K035539-022-868-G08C-DR57
TCTAATTCAGAAATCTTTTTTTTCAACCT
   
Sequence Name 03-K035539-022-868-G08C-8409
GenBank Accession LN487388 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K035539-022-868-G08C-8409
AAGCCTTTACATTTGAATATATTATGCTCGAACCGGTGGCTCCCAAAATATAAGCTGATG
CTTTGAGAAGCGTTTCGATTTCAAGCTCTTTCTCTGAATCGAGATTCACTAACGTTCCCT
TTTTTTCTTGATCGTCTAATCCTGACCGTCGATTTGGTCCGACCGGGTTCTCTTCGTCGG
ATTCGGATTCCGATGTCTCAGATTCCTCTTCTTCTTCTTCGCAGTCACCGTCAACGTAAA
CGGATTTGCGTAAACAGTACCATTTTGAGACCTTGGAATCTGTTGATGACGTGGACCATT
TTGACGTAGCCGTTACGGTCTTCCGTTTTCTCGACTGGTAAATGTAGAAAAACCAAATCC
CGA

Last Updated on 06.12.2018 16:11