Sequences from confirmation amplicon


Confirmation sequences for line 869A07 and hit At1g53430 are shown.
Line ID 869A07
Locus At1g53430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19938093
Primers used for PCR EU86 - 8474, show primer details
   
Sequence Name 04-K103845-0022-869-A07C-EU86
GenBank Accession LN487889 [GenBank]
Sequencing primer gene specific primer, EU86: 5'-GACACTCACGTTTAGCATCTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103845-0022-869-A07C-EU86
CACCATTGACACTATACACAAGTAGAAATATTTTCTTTTGTAAGTACCTTTGGTTTTTTG
GGTTCTATGAACCGAAAGGAGGGAAAAAAAGGAGACTCACGAGTTATCGGTTACTGAAGG
GTAGCTGGAGATCAAGTTCCTGTATGTTTTAACCAATAAATCTAATTAGAAAAACATACA
GAAAAGGTTCAGGTGGAAAATATGAAATAACAAGAATGCTTACACATCAAGCTGATTACA
GCTTAAAGTAGGTGGCTGAGTGAAATTGTTGTCAGATAAATCTCTGAAAACAAATCAAGA
AAATTAATGACCTCGATAAGCGCAGATGGTTTCATGGTCAATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCAGCGTTATA
   
Sequence Name 20-K103846-0022-869-A07C-8409
GenBank Accession LN495182 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K103846-0022-869-A07C-8409
ATTCGGACATGAGCCATTTACATTGAATTGACCATGAAACCATCTGCGCTTATCGAGGTC
ATTAATTTTCTTGATTTGTTTTCAGAGATTTATCTGACAACAATTTCACTCAGCCACCTA
CTTTAAGCTGTAATCAGCTTGATGTGTAAGCATTCTTGTTATTTCATATTTTCCACCTGA
ACCTTTTCTGTATGTTTTTCTAATTAGATTTATTGGTTAAAACATACAGGAACTTGATCT
CCAGCTACCCTTCAGTAACCGATAACTCGTGAGTCTCCTTTTTTTCCCTCCTTTCGGTTC
ATAGAACCCAAAAAACCAAAGGTACTTACAAAAGAAAATATTTCTACTTGTGTATAGTGT
TCAATGGTGCTTGAGAGAGGGTCTTCCATGTCCAGAAGATGCTAAACGTGAGTGT
Primers used for PCR BB89 - 8474, show primer details
   
Sequence Name 59-K118446-0022-869-A07C-BB89
GenBank Accession LN510106 [GenBank]
Sequencing primer gene specific primer, BB89: 5'-AAGTCAAACTCTGCTGCTTAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118446-0022-869-A07C-BB89
ATTTGTGTGTGTGTGTGCAGAGATTTAGCTCAACATGCTAACCGGTGTAATTCCTGACAC
ATTTCGGAATCTCGATGCATTTAACTTTATGTAAGTTTCAAATTATCAGCATGGATGGCA
TTAACATGTACTCGAAAAACTGATAGTTATTTTTGTGGCTTTAGGTTTTTGAATAATAAC
TCATTGACTGGTCCAGTTCCTCAGTTCATCATCAACAGTAAAGAAAACCTGTAAGCAAAA
CTAACTAACCAATTACCAATTTATCTGCATGCATGTCGACTCTAGAGGATCCCCAATTCC
CATGGAGTCAAAGATTCAAATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACA
GTTCATACAGAGTCTCTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGA
GCACGACACGCTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGC
AATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGC
TATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCA
TTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGG
ACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCA
AGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTC
GCAAGACCCTTCCTCTATATNAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGG
GATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
   
Sequence Name 19-K118445-0022-869-A07C-8409
GenBank Accession LN494964 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118445-0022-869-A07C-8409
CATTTCATTGAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGG
TACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGA
TAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTT
GAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCT
TTGGGACCACTGTCGGCAGAGGCATCTTCAACGATGGCCTTTCCTTTATCGCAATGATGG
CATTTGTAGGAGCCACCTTCCTTTTCCACTATCTTCACAATAAAGTGACAGATAGCTGGG
CAATGGAATCCGAGGAGGTTTCCGGATATTACCCTTTGTTGAAAAGTCTCAATTGCCCTT
TGGTCTTCTGAGACTGTATCTTTGATATTTTTGGAGTAGACAAGCGTGTCGTGCTCCACC
ATGTTGACGAAGATTTTCTTCTTGTCATTGAGTCGTAAGAGACTCTGTATGAACTGTTCG
CCAGTCTTTACGGCGAGTTCTGTTAGGTCCTCTATTTGAATCTTTGACTCCATGGGAATT
GGGGATCCTCTAGAGTCGACATGCATGCAGATAAATTGGTAATTGGTTAGTTAGTTTTGC
TTACAGGTTTTCTTTACTGTTGATGATGAACTGAGGAACTGGACCAGTCAATGAGTTATT
ATTCAAAAACCTAAAGCCACAAAAATAACTATCAGTTTTTCGAGTACATGTTAATGCCAT
CCATGCTGATAATTTGAAACTTACATAAAGTTAAATGCATCGAGATTCCGAAATGTGTCA
GGAATTACACCGGTTAGCATGTTTGAGCTTAAATCTCTGCACACACACACACAAATACTC
TGATGTGAGATCTTAATCAAACCATTAGCCTTT

Last Updated on 06.12.2018 16:11