Sequences from confirmation amplicon


Confirmation sequences for line 869E10 and hit At1g21540 are shown.
Line ID 869E10
Locus At1g21540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7550002
Primers used for PCR 30ci - 8474, show primer details
   
Sequence Name 62-K107936w-0022-869-E10C-30ci
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 30ci: 5'-ACCTGATTTCAGAAACACAAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K107936w-0022-869-E10C-30ci
TGATTTTCAATGGAAGTTTTTAATAAACCAAACCGGTATTGAACATGGATGTATTTAATA
ATACCTGAACCGGGTTCTTTAGCGGTTTACGAGACGGGTAGTTGGTTAGCATGTTGAGAA
CCATTGGTGCGGCACACATGTGAGTTACGTGATGCTTATCGATCAAGTCATAGATAGTCG
GCGCGTCGACTCTACGGGTGCAGACGTTGGTGGCTCCAACCGCCGCAGTGCCCCATGTAT
ACCCCCAACCATTGGCGTGGAACATTGGTAGAGTCCACAAGTAAACCGGCCTGTTCGGAA
AATGCCAGTCAAGCAATGAGCTTACGGTTAGCATGAAAATTGCTCTGTGGCTCAACACTA
CTCCTTTGGGAGATGACGTTGTTCCAGAAGTATAGTTGAGAATCATAGGCTGCCACTCGG
TTTGAGGACGGATCCACTTGAATCTTGAATCTCCATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 59-K107935w-0022-869-E10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K107935w-0022-869-E10C-8409
TTGAAATGGAGATTCAGATTCAAGTGGATCCGTCCTCAAACCGAGTGGCAGCCTATGATT
CTCAACTATACTTCTGGAACAACGTCATCTCCCAAAGGAGTAGTGTTGAGCCACAGAGCA
ATTTTCATGCTAACCGTAAGCTCATTGCTTGACTGGCATTTTCCGAACAGGCCGGTTTAC
TTGTGGACTCTACCAATGTTCCACGCCAATGGTTGGGGGTATACATGGGGCACTGCGGCG
GTTGGAGCCACCAACGTCTGCACCCGTAGAGTCGACGCGCCGACTATCTATGACTTGATC
GATAAGCATCACGTAACTCACATGTGTGCCGCACCAATGGTTCTCAACATGCTAACCAAC
TACCCGTCTCGTAAACCGCTAAAGAACCCGGTTCAGGTATTATTAAATACATCCATGTTC
AATACCGGTTTGGTTTATTAAAAACTTCCATTTGAAAATCATTTGGTGAACTTTTGAATA
AACTCTGTTTTGTGTTTCTAAAATCAGG

Last Updated on 06.12.2018 16:11