Sequences from confirmation amplicon


Confirmation sequences for line 869F06 and hit At4g34131 are shown.
Line ID 869F06
Locus At4g34131 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16343565
Primers used for PCR NA80 - 8474, show primer details
   
Sequence Name 67-K117042-0022-869-F06C-NA80
GenBank Accession LN511893 [GenBank]
Sequencing primer gene specific primer, NA80: 5'-CTCTTCTCTGCCATATTCTCTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K117042-0022-869-F06C-NA80
ATCGTTGAAGAAACCCTCCTTCCCGGCAGCTTTAGCCATCTCTGCCAACTTCTTTGCCCT
CTCCCGCCTCTCATCCGCCTCTTCCCCAACCAACACCTCCCTCACCGCTTTAACCACTTT
CTCTCTGCTAATGAAATCTCCCGTAGTTCTTACATTCTTTTTCGCTCCCACGCTCACTCC
TGTTCTGAGCACTTGCGTAACCAATTTCTCATTGTAGAATTGCTCCGCTGCTACAGGCCA
TGTCACCATTGGTAGCCCTGCATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 37-K117041-0022-869-F06C-8409
GenBank Accession LN502757 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117041-0022-869-F06C-8409
TTGAATATACATGCAGGGCTACCAATGGTGACATGGCCTGTAGCAGCGGAGCAATTCTAC
AATGAGAAATTGGTTACGCAAGTGCTCAGAACAGGAGTGAGCGTGGGAGCGAAAAAGAAT
GTAAGAACTACGGGAGATTTCATTAGCAGAGAGAAAGTGGTTAAAGCGGTGAGGGAGGTG
TTGGTTGGGGAAGAGGCGGATGAGAGGCGGGAGAGGGCAAAGAAGTTGGCAGAGATGGCT
AAAGCTGCCGTGGAAGGAGGGTCTTCTTTCAACGATCTAAACAGCTTCATAGAAGAGTTT
ACCTCGTAATAAGCAGAGAATATGGCAGAGAAGAGA

Last Updated on 06.12.2018 16:11