Sequences from confirmation amplicon


Confirmation sequences for line 869H11 and hit At2g03370 are shown.
Line ID 869H11
Locus At2g03370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1025899
Primers used for PCR 88yp - 8474, show primer details
   
Sequence Name 29-K122428-0022-869-H11C-88yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 88yp: 5'-CTAAATATGATTACCTCGAACCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K122428-0022-869-H11C-88yp
TATCCTCGACCATGTTACATATCGACTACCAACATGAGACGAGGCTTGTGGGTTTTAGGA
GTAGATATGTTTGTGGTGAAAGCTTTTTCTAAAAGATTGTGGAAGTCCACTAATGATTTG
GAGTTGGGTATTTGGGTTGGGTCTATTGTCATGGGCCAATGTGTAATTAGGCCCACAATA
GCTGAAGTGAAGCAATGTGTAGTAGCAACACTTTCTTTGTCTAGTAGTAATATGGGTTTA
TGTTTGCTAAACGTACCTAGAATATCGATGTATTTTGGCATCCACCATTCCTTTGCATTT
ACTACCACCGGGATGAAATCACGGTCCGGATATACCGAATTGGCCGTGATGAAAAGCGGG
ATAAAACCATCCATTAAATCGTGATAGATGCTTCCGGTGTAACCTCCGGCACTGAACACA
ATCGCCGGCAAATCATGTGTTATGTCACATGATCGGGGCAGACCCAATGGGCCTGAGGTT
AGTGTTAGCTCTCTGATCCTAGGCATGATCCAGTTGTCAGCCTTTTTTGGGTATGGCCTG
ATCTTTTCGACAAGTGGTGCTGATGTTGGGTCCATTAGAGTCAAAGTTCCGGTTTTAAGG
TCCAGATTACATGAGCCGTTGATTGAGCAGAGATCGTAATTAGTGTGGGATCGGTCACAT
GTGATTGTTGCCGAAGTAGTCTCTTGCGGTTGTTGCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 05-K122427-0022-869-H11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K122427-0022-869-H11C-8409
AATTGAATATATCCTGCACAACCGCAAGAGACTACTTCGGCAACAATCACATGTGACCGA
TCCCACACTAATTACGATCTCTGCTCAATCAACGGCTCATGTAATCTGGACCTTAAAACC
GGAACTTTGACTCTAATGGACCCAACATCAGCACCACTTGTCGAAAAGATCAGGCCATAC
CCAAAAAAGGCTGACAACTGGATCATGCCTAGGATCAGAGAGCTAACACTAACCTCAGGC
CCATTGGGTCTGCCCCGATCATGTGACATAACACATGATTTGCCGGCGATTGTGTTCAGT
GCCGGAGGTTACACCGGAAGCATCTATCACGATTTAATGGATGGTTTTATCCCGCTTTTC
ATCACGGCCAATTCGGTATATCCGGACCGTGATTTCATCCCGGTGGTAGTAAATGCAAAG
GAATGGTGGATGCCAAAATACATCGATATTCTAGGTACGTTTAGCAAACATAAACCCATA
TTACTACTAGACAAAGAAAGTGTTGCTACTACACATTGCTTCACTTCAGCTATTGTGGGC
CTAATTACACATTGGCCCATGACAATAGACCCAACCCAAATACCCAACTCCAAATCATTA
GTGGACTTCCACAATCTTTTAGAAAAAGCTTTCACCACAAACATATCTACTCCTAAAACC
CACAAGCCTCGTCTCATGTTGGTTAGTCGATATGGTAACATTGGTCGAGTGATATTAAAC
GAGCAAGAGATCAAAGAGATGCTTGAAGATGTCGGGTTCGAGGT

Last Updated on 06.12.2018 16:11