Sequences from confirmation amplicon


Confirmation sequences for line 870A03 and hit At4g35050 are shown.
Line ID 870A03
Locus At4g35050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16683930
Primers used for PCR JF14 - 8474, show primer details
   
Sequence Name 43-K102502-0022-870-A03C-8409
GenBank Accession LN504792 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K102502-0022-870-A03C-8409
GTTAAAGATAACCCTCCTTAAGGAACTCCAAGCCAATCCATAACCTTCTTGTTCATGTCC
CATTAGCCTCAGATCAGGATCACACTCACTTGTTTGGGGCTTTCCAGAGAGTCTGGCGTA
ATCAAACAGAAACACCTCAGAGCCACTTGTTTTAGCACCCACAAGAGTTGGCTTTTGTGG
CATACACCTCGCTCTGTTCACTTCTCCATCAACACGTATCTTCTGCTTAATCTCCACCTG
CAAATGATAGAGTTTTCAGAAACTCAGTCTCCAATAAGTTTTCACATATTTTCAAGTGCC
TAAGAAATTGTCCGAATTTATATAAAAGCTGAATTCATGAATATTTTCGATAAGGAAAAG
GCTAGTCCTTTTGTAGAACACTCAAGACAGTTGTTGATTCCGTCCTCACTCAAAGCTAGA
ACTTTCGTCGAACACATGGTGTGCACAGAACACAACTTATAAAAAATCCGCAGAAAGTGA
AGAAATCTCCAGGAGTAAATTACCTTAGGGACGATAGGTTCCTGATCTCTTCCACCTAAT
CCTGGTTCAGCGTCGGGTGTCGGAATAACGACATCGGCGACCATTAGAAAATCTTGGGCA
CCACCGGAGGTATGAGTCCCAAGGATAAGCTTATGGACGGCAAAGTAAGGATCTTTCGAG
TACGGAATCGGCGTCGAAGGAACCCAGTGGAGAGTAAGAGAAGGCCATTCTAGTGGGTGA
GAGATCATAAGATCGTAGAG
   
Sequence Name 43-K102503-0022-870-A03C-JF14
GenBank Accession LN504793 [GenBank]
Sequencing primer gene specific primer, JF14: 5'-AAGGAACACACCTTTTCTCTACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K102503-0022-870-A03C-JF14
GCCTTCTCTTACTCTCCACTGGGTTCCTTCGACGCCGATTCCGTACTCGAAAGATCCTTA
CTTTGCCGTCCATAAGCTTATCCTTGGGACTCATACCTCCGGTGGTGCCCAAGATTTTCT
AATGGTCGCCGATGTCGTTATTCCGACACCCGACGCTGAACCAGGATTAGGTGGAAGAGA
TCAGGAACCTATCGTCCCTAAGGTAATTTACTCCTGGAGATTTCTTCACTTTCTGCGGAT
TTTTTATAAGTTGTGTTCTGTGCACACCATGTGTTCGACGAAAGTTCTAGCTTTGAGTGA
GGACGGAATCAACAACTGTCTTGAGTGTTCTACAAAAGGACTAGCCTTTTCCTTATCGAA
AATATTCATGAATTCAGCTTTTATATAAATTCGGACAATTTCTTAGGCACTTGAAAATAT
GTGAAAACTTATTGGAGACTGAGTTTCTGAAAACTCTATCATTTGCAGGTGGAGATTAAG
CAGAAGATACGTGTTGATGGAGAAGTGAACAGAGCGAGGTGTATGCCACAAAAGCCAACT
CTTGTGGGTGCTAAAACAAGTGGCTCTGAGGTGTTTCTGTTTGATTACGCCAGACTCTCT
GGAAAGCCCCAAACAAGTGAGTGTGATCCTGATCTGAGGCTAATGGGACATGAACAAGAA
GGTTATGGATTGGCTTGGAGTTCCTTTAAGGAGGGTTATCTTTTGAACCCAATGCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTTCAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACCAAATACGATCCGTCGTATTTATAGGCGAAAGCAATAAC
AAATTATTCTAATT

Last Updated on 06.12.2018 16:11