Sequences from confirmation amplicon


Confirmation sequences for line 870B10 and hit At5g48970 are shown.
Line ID 870B10
Locus At5g48970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19858790
Primers used for PCR CN72 - 8474, show primer details
   
Sequence Name 26-K104153-0022-870-B10C-CN72
GenBank Accession LN497898 [GenBank]
Sequencing primer gene specific primer, CN72: 5'-CTTTGGATTGTATTGTGGAGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104153-0022-870-B10C-CN72
TGGTAGCTTTTAATATTTAGAGATGGTTGAAGAATGTTATATGTGATTGCAGGTTCAGCT
AGAACCGACGACTTCATGGGGTTTGGTTCGGGGAAACTTGTCAGGAGCTTCTAAGTATAC
TGGAATGGTACAGGCGACTAAAGATATATTTAGAGAAGAAGGTTTTCGGGTCCGTCTCTT
GAACATTTCCGTTTTCTGAAACCATTTCTTTTTTGTTTATAGTATGTTAGTAGTTTCAGG
TTTGTGGCATTAGTTTTTATGAGGTCTTGTTCTCTGTTTTGATGTTTGATACTTGTTTTC
TTTATAAATGGCTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGC
   
Sequence Name 26-K104154-0022-870-B10C-8409
GenBank Accession LN497899 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104154-0022-870-B10C-8409
GCCATTTACATTGAATAAGCCATTTATAAAGAAAACAAGTATCAAACATCAAAACAGAGA
ACAAGACCTCATAAAAACTAATGCCACAAACCTGAAACTACTAACATACTATAAACAAAA
AAGAAATGGTTTCAGAAAACGGAAATGTTCAAGAGACGGACCCGAAAACCTTCTTCTCTA
AATATATCTTTAGTCGCCTGTACCATTCCAGTATACTTAGAAGCTCCTGACAAGTTTCCC
CGAACCAAACCCCATGAAGTCGTCGGTTCTAGCTGAACCTGCAATCACATATAACATTCT
TCAACCATCTCTAAATATATAAAAGCTAACCATTTTCTAACAATGATAATTCTCATCTAA
AACCTCCACAATACNANTCCAAA

Last Updated on 06.12.2018 16:11