Sequences from confirmation amplicon


Confirmation sequences for line 870C11 and hit At2g23350 are shown.
Line ID 870C11
Locus At2g23350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9945120
Primers used for PCR 49bk - 8474, show primer details
   
Sequence Name 54-K107839-0022-870-C11C-49bk
GenBank Accession LN508500 [GenBank]
Sequencing primer gene specific primer, 49bk: 5'-AGTGGTACTAGCAAAGGATCAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K107839-0022-870-C11C-49bk
GAGCTCAGAGTGGTAATTTAATAATCCTGTGTCAAGACAATATTAAATTTGTTTTGAGCC
TCTATTTTCTTTCTTGATTCAATTTCTTTTGGGGTCTTCTGCAGCTGAATGAAATGAATG
GTAAAATGGTTGGTGGCAAACCGTTGTATGTTGCTCTTGCACAGAGGAAAGAAGAAAGGA
GGGCTAAGCTGCAGGTAGTACTTCCCACCATAGATAAACAACCCCTACGTACACTTATGT
TTGCTATGTCTCAAGTCCTTATGTTTCTTTTTCAGGCACAGTTTTCTCAAATGAGACCTG
CTTTTATCCCCGGTGTGGGTCCTCGAATGCCAATATTTACAGGTGGTGCTCCAGGTCTTG
GACAACAGATTTTTTACGCATTGTAAATGGTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 86-K107840-0022-870-C11C-8409
GenBank Accession LN514663 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K107840-0022-870-C11C-8409
GCATTTACATTGAATTATCCTGACCATTTACAATGCGTAAAAAATCTGTTGTCCAAGACC
TGGAGCACCACCTGTAAATATTGGCATTCGAGGACCCACACCGGGGATAAAAGCAGGTCT
CATTTGAGAAAACTGTGCCTGAAAAAGAAACATAAGGACTTGAGACATAGCAAACATAAG
TGTACGTAGGGGTTGTTTATCTATGGTGGGAAGTACTACCTGCAGCTTAGCCCTCCTTTC
TTCTTTCCTCTGTGCAAGAGCAACATACAACGGTTTGCCACCAACCATTTTACCATTCAT
TTCATTCAGCTGCAGAAGACCCCAAAAGAAATTGAATCAAGAAAGAAAATAGAGGCTCAA
AACAAATTTAATATTGTCTTGACACAGGATTATTTAAATTACCACTCTTGAAGCTTCACT
GGCAGCAGAGAAGGCAACAAATCCTGATCCTTTGCTAGTACCAC

Last Updated on 06.12.2018 16:11