Sequences from confirmation amplicon


Confirmation sequences for line 870F05 and hit At3g22740 are shown.
Line ID 870F05
Locus At3g22740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8034229
Primers used for PCR KQ64 - 8474, show primer details
   
Sequence Name 25-K101500-0022-870-F05C-8409
GenBank Accession LN497375 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K101500-0022-870-F05C-8409
CATTTACATTGACCAAAACTTTTGTTATGACTATGATTTTTCCAATTTTATACACAAATG
TAACAGACAAAAATGACTAGGATCGATCAAAGAACCTGTATTCGGAGCCGTCAGCGAGAT
AAGCTCCATAGCTACCAACGGAAGCTGCGACAAGAATTGGTCTCCGGGAAGCTTTTCCAG
CGTAGGCGAAATCCCATGAACCCTTGGTGCATCTGTTGTAGAAAATCTCACGTGCCTCGT
ACGTGATTTCAACGCTCCTTCTAAGTAAATTTTCTGCTTCTCCCACCGATAGACCTTTTG
CCACAAAACCTTGTATCGTCGCCTGTATAATAAATTAATTTGTTTAAGTACCTTGATGCA
TTTTGAAACTAATTTCCTTTTTTTTACCACATTTACTTCAAGCAAACAAACCAAATCAAA
CTATTTAGTTAATATAGGCCAAAGTACCTGGTAAGATGCTGTTATG
   
Sequence Name 25-K101504-0022-870-F05C-KQ64
GenBank Accession LN497376 [GenBank]
Sequencing primer gene specific primer, KQ64: 5'-TCATCATAACAGCATCTTACCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K101504-0022-870-F05C-KQ64
TAGTTTGATTTGGTTTGTTTGCTTGAAGTAATGTGGTAAAAAAAAGGAAATTAGTTTCAA
AATGCATCAAGGTACTTAAACAAATTAATTTATTATACAGGCGACGATACAAGGTTTTGT
GGCAAAAGGTCTATCGGTGGGAGAAGCAGAAAATTTACTTAGAAGGAGCGTTGAAATCAC
GTACGAGGCACGTGAGATTTTCTACAACAGATGCACCAAGGGTTCATGGGATTTCGCCTA
CGCTGGAAAAGCTTCCCGGAGACCAATTCTTGTCGCAGCTTCCGTTGGTAGCTATGGAGC
TTATCTCGCTGACGGCTCCNAATACAGGTTCTTTGATCGATCCTAGTCATTTTTGTCTGT
TACATTTGTGTATAAAATTGGAAAAATCATAGTCATAACAAAAGTTTTGGTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11