Sequences from confirmation amplicon


Confirmation sequences for line 870H12 and hit At2g17950 are shown.
Line ID 870H12
Locus At2g17950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7810207
Primers used for PCR BD49 - 8474, show primer details
   
Sequence Name 50-K028245-022-870-H12C-8409
GenBank Accession LN507056 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K028245-022-870-H12C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCATAACAAGCCATA
TCCCAGCTTCAATAACGGTGACTAATCTCTCTTATACATGCTTTTTTATGAATTTATTCA
GGTGGTTTTTTATAATTACTAAGAGAACCATTTACACCACTAGCTATTACATGAATTAGC
ACACACAAAAAAAAAAAAACTAACAAGATCTACATATATGACTTTGATAGATATATGTAT
ATGTTAATTGTTAAAAAAATTTTAAAAAAGGCTGTTGATTTATGAATTTATTCAAAGGGT
TTTGATTTAAAAATCTAAAATATATATTATAGGAACATGTTACACCCCATCTATTATTAC
ATGAATTAGTAAAAATAATTAACCCCAAAAAAATGTTATGTACATGTAAGGACGTATATT
TGAAGTTATGGATCTTGATGGGGGCAAACCAAATTTGCATCTAAAAAACGGTTTCTTTGT
TGTGTAAGGGAAACTAGACTCACGGGCATTTTATTTTCATTTGGGACTTGAAACACAATT
AGGGATTTGGGTTAGTAGAAAAAAAGACACAAATTTAAGGTCTCTTTCT
   
Sequence Name 51-K028245-022-870-H12C-BD49
GenBank Accession LN507388 [GenBank]
Sequencing primer gene specific primer, BD49: 5'-GGGGAAAAGAATGAAGAAAGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K028245-022-870-H12C-BD49
ATAAAATTAGTGATTTTTTTTCTACTAACCCAAATCCCTAATTGTGTTACAAGTCACAAA
TGAAAATAAAATGCACGTGAGTCTAGTTTCCATTACACAACAAAGAAACCGTTCTTTAGA
TGCAAATTTGGTTTGCCCCAATCAAGATCCATAACTTCAAATATACGTCATTACATGTAC
ATAACATCTTCTTGGGTTTAATTATTTTTACTAATTCATGTAATAATAGATGGTGTGTAA
CATGTTCCTATAATATATATTTTAGATTTTTAAATCAAAACCATCTGAATAAATTCATAA
ATCAACAGCATTTTTTAAAATCTTTTTAACAATTAACATATACATATATCTATCAAAGTC
ATATATGTAGATCTTGTTAGTTTTTTTTTTTTTGGGGGGGCAAATTCAGGTAAAACCTAG
GGGGGAAAAGGGTTCTCTTAGTAATTATAAAAAACCCCCTGAATAAATTCATAAAAAAGC
ATGTATAAAAAAGATTAGTCCCCGTTTTTGAACCTGGAATATGGCTTGTTATGACAGGAT
ATATTCAATTGAAAAGGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGA
GGGTCAATTTGGAAAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAA

Last Updated on 06.12.2018 16:11